A nanocatalyst-based assay for proteins:: DNA-free ultrasensitive electrochemical detection using catalytic reduction of p-nitrophenol by gold-nanoparticle labels

被引：275
作者：
Das, Jagotamoy [1 ]
Aziz, Md. Abdul [1 ]
Yang, Haesik [1 ]
机构：
[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2006年 / 128卷 / 50期
关键词：
D O I：
10.1021/ja0672167
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
This communication reports a nanocatalyst-based electrochemical assay for proteins. Ultrasensitive detection has been achieved by signal amplification combined with noise reduction: the signal is amplified both by the catalytic reduction of p-nitrophenol to p-aminophenol by gold-nanocatalyst labels and by the chemical reduction of p-quinone imine to p-aminophenol by NaBH4; the noise is reduced by employing an indium tin oxide electrode modified with a ferrocenyl-tethered dendrimer and a hydrophilic immunosensing layer. Copyright © 2006 American Chemical Society.
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页码：16022 / 16023
页数：2
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