Overexpression of the Arabidopsis anaphase promoting complex subunit CDC27a increases growth rate and organ size

被引：51

作者：

Rojas, Cristian Antonio ^{[2
]}

Eloy, Nubia Barbosa ^{[2
,4
]}

Lima, Marcelo de Freitas ^{[2
]}

Rodrigues, Roberta Lopes ^{[2
]}

Franco, Luciana Ozorio ^{[1
]}

Himanen, Kristiina ^{[3
,4
]}

Beemster, Gerrit T. S. ^{[3
,4
]}

Hemerly, Adriana Silva ^{[1
,2
]}

Gomes Ferreira, Paulo Cavalcanti ^{[1
,2
]}

机构：

[1] Diretoria Pesquisa Cient, Inst Pesquisas Jardim Bot Rio de Janeiro, BR-22460030 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Bioquim Med, BR-21941590 Rio De Janeiro, Brazil

[3] VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium

[4] Univ Ghent, Dept Mol Genet, B-9052 Ghent, Belgium

来源：

PLANT MOLECULAR BIOLOGY | 2009年 / 71卷 / 03期

关键词：

APC; Cell cycle; Development; Arabidopsis; CYCLIN-DEPENDENT KINASES; PLANT-CELL CYCLE; TRANSCRIPTION FACTOR; GENE-EXPRESSION; DIFFERENTIATION; THALIANA; DIVISION; TRANSFORMATION; PROLIFERATION; REPLICATION;

D O I：

10.1007/s11103-009-9525-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Anaphase Promoting Complex (APC) controls CDK activity by targeting the ubiquitin-dependent proteolysis of S-phase and mitosis-promoting cyclins. Here, we report that the ectopic expression of the Arabidopsis CDC27a, an APC subunit, accelerates plant growth and results in plants with increased biomass production. CDC27a overexpression was associated to apical meristem restructuration, protoplasts with higher H-3-thimidine incorporation and altered cell-cycle marker expression. Total protein extracts immunoprecipitated with a CDC27a antibody showed ubiquitin ligase activity, indicating that the Arabidopsis CDC27a gets incorporated into APC complexes. These results indicate a role of AtCDC27a in regulation of plant growth and raise the possibility that the activity of the APC and the rates of plant cell division could be regulated by the concentration of the CDC27a subunit.

引用

页码：307 / 318

页数：12

共 40 条

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