Fructosyltransferase production by Aspergillus oryzae BM-DIA using solid-state fermentation and the properties of its nucleotide and protein sequences

被引：9

作者：

Michel, Mariela R. ^{[1
]}

Flores-Gallegos, Adriana C. ^{[1
]}

Villarreal-Morales, Sandra L. ^{[1
]}

Aguilar-Zarate, Pedro ^{[2
]}

Aguilar, Cristobal N. ^{[1
]}

Riutort, Marta ^{[3
]}

Rodriguez-Herrera, Raul ^{[1
]}

机构：

[1] Autonomous Univ Coahuila, Sch Chem, Dept Food Res, Blvd V Carranza & Ing,Jose Cardenas S-N, Saltillo 25280, Coahuila, Mexico

[2] Inst Tecnol Ciudad Valles, Tecnol Nacl Mexico, Dept Engn, Carr Ingenio Plan Ayala Km 2, Ciudad Valles 79010, San Luis Potosi, Mexico

[3] Univ Barcelona, Dept Genet, Fac Biol, Ave Diagonal,643, Barcelona 08028, Spain

来源：

FOLIA MICROBIOLOGICA | 2021年 / 66卷 / 03期

关键词：

Fructooligosaccharides; Agave sap; Amino acids; Nucleotide sequencing; FRUCTOOLIGOSACCHARIDES PRODUCTION; GENE CLONING; ENZYMES; AGUAMIEL; JAPONICUS; INFERENCE; MRBAYES;

D O I：

10.1007/s12223-021-00862-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fructosyltransferase (FTase) catalyzes the transfer of a fructosyl group to a sucrose molecule or a fructooligosaccharide (FOS) when a FOS with a longer chain is formed. Production of FTase by two Aspergillus species and its mixture was exploited using solid-state fermentation (SSF) and employing agave sap as substrate. The maximum FTase activity (1.59 U/mL) by Aspergillus oryzae was obtained after 24 h, using a temperature of 30 degrees C, with an inoculum of 2 x 10(7) spores/mL. The nucleotide sequence coding for the fructosyltransferase showed 1494 bp and encodes for a protein of 498 amino acids. The hypothetical molecular tertiary structure of Aspergillus oryzae BM-DIA FTase showed the presence of structural domains, such as a five-bladed beta-propeller domain characteristic of GH (glycoside hydrolase) and C terminal, which forms a beta-sandwich module. This study contributes to the knowledge of stability, compatibility, and genetic expression of Aspergillus oryzae BM-DIA under SSF bioprocess conditions for industrial production of fructosyltransferase.

引用

页码：469 / 481

页数：13

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