Abundance and turnover of GLP-1 producing L-cells in ileal mucosa are not different in patients with and without type 2 diabetes

Introduction. The gastrointestinal hormone GLP-1 is released from enteroendocrine L cells and augments postprandial insulin secretion. In patients with type 2 diabetes, the incretin effect is markedly diminished. It is unclear, whether this is due to a reduction in the abundance of L-cells in the intestine. Methods. Ileal tissue samples from 10 patients with and 10 patients without diabetes that underwent surgery for the removal of colon tumors were included. Tissue sections were stained for GLP-1, Ki67, TUNEL and chromogranin A. Results. The number of L-cells was not different between patients with and without diabetes in either crypts (1.81 +/- 0.21% vs. 1.49 +/- 0.24%, respectively; p = 0.31) or villi (1.07 +/- 0.16% vs. 0.83 +/- 0.10%, respectively; p = 0.23). L-cell number was higher in crypts than in villi (p < 0.0001). L-cell replication was detected rarely and not different between the groups. L-cell apoptosis was similar in patients with and without diabetes in both crypts (7.84 +/- 2.77% vs. 8.65 +/- 3.77%, p = 0.85) and villi (4.48 +/- 2.89% vs. 8.62 +/- 4.64%, p = 0.42). Chromogranin A staining was found in a subset of L-cells only. Conclusions. Intestinal L-cell density is higher in crypts than in villi. Chromogranin A is not a prerequisite for GLP-1 production. L-cell density and turnover are not different between patients with and without diabetes. Thus, alterations in the number of GLP-1 producing cells do not explain the reduced incretin effect in patients with type 2 diabetes. (C) 2015 Elsevier Inc. All rights reserved.