A new mechanism of mammalian cell resistance to the toxic effects of DNA-binding agents

被引:1
|
作者
Levina, V [1 ]
Varfolomeeva, E [1 ]
Sukhareva, E [1 ]
Shabalina, E [1 ]
Drobchenko, E [1 ]
Filatov, M [1 ]
机构
[1] Petersburg Nucl Phys Inst, St Petersburg 188350, Russia
来源
ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2000年 / 28卷 / 03期
关键词
multidrug resistance; DNA clearing; mammalian cells; Hoechst; 33342; mitomycin C;
D O I
10.1177/026119290002800308
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The phenomenon of active dissociation of the noncovalently binding vital dye Hoechst 33342 from DNA in living cells (DNA clearing) is described. Step-by-step selection with increasing concentrations of the dye resulted in a series of rodent cell lines that were resistant to the toxic action of Hoechst 33342. Some of the lines exhibited enhanced dissociation of the bisbenzimidazole dye-DNA complex. Two cell lines from this group (AA8HoeR-7 and LHoeR-3) were analysed in detail and compared with a Syrian hamster tumour cell line, a typical example of mdr-I-mediated multidrug-resistant cell lines. The markedly enhanced level of DNA clearing in AA8HoeR-7 and LHoeR-3 cells leads to high cellular resistance to the toxic effect of Hoechst 33343 and cross-resistance to mitomycin C, a minor-groove alkylating agent in clinical use. Our results suggest that DNA clearing is one of the mechanisms of multidrug resistance in tumour cells.
引用
收藏
页码:451 / 456
页数:6
相关论文
共 50 条
  • [31] A SEARCH FOR POTENTIAL ANTITUMOR AGENTS - BIOLOGICAL EFFECTS AND DNA-BINDING OF A SERIES OF ANTHRAQUINONE DERIVATIVES
    PALU, G
    PALUMBO, M
    ANTONELLO, C
    MELONI, GA
    MARCIANIMAGNO, S
    MOLECULAR PHARMACOLOGY, 1986, 29 (02) : 211 - 217
  • [32] SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
    Lopez-Mosqueda, Jaime
    Maddi, Karthik
    Prgomet, Stefan
    Kalayill, Sissy
    Marinovic-Terzic, Ivana
    Terzic, Janos
    Dikic, Ivan
    ELIFE, 2016, 5
  • [33] DETECTION OF A DNA-BINDING FACTOR ASSOCIATED WITH MAMMALIAN DNA POLYMERASE-ALPHA
    MECHALI, M
    DERECONDO, AM
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1978, 82 (01) : 255 - 264
  • [34] COMPOSITION AND DNA-BINDING PROPERTIES OF THE NUCLEAR MATRIX PROTEINS FROM MAMMALIAN-CELL NUCLEI
    MULLENDERS, LHF
    EYGENSTEYN, J
    BROEN, A
    WANKA, F
    BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 698 (01) : 70 - 77
  • [35] Design of new polypyridyl ligands and their effects on DNA-binding mechanisms of complexes
    Zhang, QL
    Liu, JH
    Liu, JZ
    Ren, XZ
    Zhang, PX
    Wei, B
    Xu, H
    Ji, LN
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2003, 24 (10): : 1753 - 1755
  • [36] EFFECTS OF COUNTERSTAINING WITH DNA-BINDING DRUGS ON FLUORESCENT BANDING-PATTERNS OF HUMAN AND MAMMALIAN CHROMOSOMES
    SCHNEDL, W
    DANN, O
    SCHWEIZER, D
    EUROPEAN JOURNAL OF CELL BIOLOGY, 1980, 20 (03) : 290 - 296
  • [37] EFFECTS OF DNA-BINDING PROTEINS ON DNA METHYLATION INVITRO
    KAUTIAINEN, TL
    JONES, PA
    BIOCHEMISTRY, 1985, 24 (05) : 1193 - 1196
  • [38] MAMMALIAN DNA METHYLASE - BINDING STUDIES AND MECHANISM
    MORRIS, NR
    DRAHOVSK.D
    FEDERATION PROCEEDINGS, 1971, 30 (03) : 1314 - &
  • [39] DNA-binding mechanism of spiropyran photoswitches: the role of electrostatics
    Avagliano, Davide
    Sanchez-Murcia, Pedro A.
    Gonzalez, Leticia
    PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2019, 21 (17) : 8614 - 8618
  • [40] POSSIBLE MECHANISM FOR DONOR DNA-BINDING AND TRANSPORT IN HEMOPHILUS
    KAHN, ME
    MAUL, G
    GOODGAL, SH
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (20): : 6370 - 6374