A systematic RNA interference screen reveals a cell migration gene network in C-elegans

被引：65

作者：

Cram, Erin J. ^{[1
]}

Shang, Hongyu ^{[1
]}

Schwarzbauer, Jean E. ^{[1
]}

机构：

[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

JOURNAL OF CELL SCIENCE | 2006年 / 119卷 / 23期

关键词：

cell migration; Caenorhabditis elegans; RNAi; gonad;

D O I：

10.1242/jcs.03274

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cell migration is essential during embryonic development and tissue morphogenesis. During gonadogenesis in the nematode Caenorhabditis elegans, migration of the distal tip cells forms two U-shaped gonad arms. Malformation results if the distal tip cells stop prematurely or follow an aberrant path, and abnormalities are easily visualized in living nematodes. Here we describe the first comprehensive in vivo RNA interference screen for genes required for cell migration. In this non-biased screen, we systematically analyzed 16,758 RNA-interference depletion experiments by light microscopy and identified 99 genes required for distal tip cell migration. Genetic and physical interaction data connect 59 of these genes to form a cell migration gene network that defines distal tip cell migration in vivo.

引用

页码：4811 / 4818

页数：8

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