Long-term terbutaline exposure stimulates α1-Na+K+-ATPase expression at posttranscriptional level in rat fetal distal lung epithelial cells

Rahman MS, Gandhi S, Otulakowski G, Duan W, Sarangapani A, O'Brodovich H. Long-term terbutaline exposure stimulates alpha(1)-Na+-K+-ATPase expression at posttranscriptional level in rat fetal distal lung epithelial cells. Am J Physiol Lung Cell Mol Physiol 298: L96-L104, 2010. First published October 30, 2009; doi:10.1152/ajplung.00158.2009.-Transepithelial Na+ transport through epithelial Na+ channels (ENaC) on the apical membrane and Na+-K+-ATPase activity on the basolateral membrane of distal lung epithelial cells are critical for alveolar fluid clearance. Acute exposure to beta-adrenergic agonists stimulates lung fluid clearance by increasing Na+ transport. We investigated the effects of chronic exposure to the beta(2)-adrenergic agonist terbutaline on the transepithelial Na+ transport in rat fetal distal lung epithelia ( FDLE). FDLE monolayers exposed to 10(-4) M terbutaline for 48 h had significantly increased propanolol-blockable transepithelial total and amiloride-sensitive short-circuit current (I-sc); however, when these chronically exposed monolayers were acutely exposed to additional beta-agonists and intracellular cAMP upregulators, there was no further increase in I-sc. Monolayers exposed to terbutaline for >48 h had I-sc similar to control cells. Ouabain-sensitive Na+-K+-ATPase activity was increased in 48-h terbutaline-exposed FDLE whose apical membranes were permeabilized with nystatin. In contrast, terbutaline did not increase amiloride-sensitive apical membrane I-sc in FDLE whose basolateral membranes were permeabilized with nystatin. Terbutaline treatment did not affect alpha-, beta-, or gamma-ENaC mRNA or alpha-ENaC protein steady-state levels, but increased total cellular levels and rate of synthesis of alpha(1)-Na+-K+-ATPase protein in FDLE in the absence of any change in alpha(1)-Na+-K+-ATPase mRNA. Total cellular alpha(1)-Na+-K+-ATPase mRNA and protein levels were not affected by terbutaline. These data suggest that FDLE have different responses from adult type II epithelial cells when chronically exposed to terbutaline, and their increased transepithelial Na+ transport occurs via a posttranscriptional increase in alpha(1)-Na+-K+-ATPase expression.