Synonymous single nucleotide polymorphism in arsenic (+3) methyltransferase of the Western mosquitofish (Gambusia affinis) and its gene expression among field populations

被引:3
|
作者
Park, Daesik [1 ]
Propper, Catherine R. [2 ]
Wang, Guangning [2 ]
Salanga, Matthew C. [2 ]
机构
[1] Kangwon Natl Univ, Div Sci Educ, Chunchon 24341, Kangwon, South Korea
[2] No Arizona Univ, Dept Biol Sci, Box 5640, Flagstaff, AZ 86011 USA
基金
美国国家卫生研究院;
关键词
Arsenic; as3mt; Cyt19; mosquitofish; Gambusia affinis; Arizona; AS3MT; EXPOSURE; IDENTIFICATION; METHYLATION; ADAPTATION;
D O I
10.1007/s10646-021-02376-8
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Naturally occurring arsenic is toxic at extremely low concentrations, yet some species persist even in high arsenic environments. We wanted to test if these species show evidence of evolution associated with arsenic exposure. To do this, we compared allelic variation across 872 coding nucleotides of arsenic (+3) methyltransferase (as3mt) and whole fish as3mt gene expression from three field populations of Gambusia affinis, from water sources containing low (1.9 ppb), medium-low (3.3 ppb), and high (15.7 ppb) levels of arsenic. The high arsenic site exceeds the US EPA's Maximum Contamination Level for drinking water. Medium-low and high populations exhibited homozygosity, and no sequence variation across all animals sampled. Eleven of 24 fish examined (45.8%) in the low arsenic population harbored synonymous single nucleotide polymorphisms (SNPs) in exons 4 and/or 10. SNP presence in the low arsenic population was not associated with differences in as3mt transcript levels compared to fish from the medium-low site, where SNPs were noted; however, as3mt expression in fish from the high arsenic concentration site was significantly lower than the other two sites. Low sequence variation in fish populations from sites with medium-low and high arsenic concentrations suggests greater selective pressure on this allele, while higher variation in the low population suggests a relaxed selection. Our results suggest gene regulation associated with arsenic detoxification may play a more crucial role in influencing responses to arsenic than polymorphic gene sequence. Understanding microevolutionary processes to various contaminants require the evaluation of multiple populations across a wide range of pollution exposures.
引用
收藏
页码:711 / 718
页数:8
相关论文
共 48 条
  • [31] A non-synonymous single-nucleotide polymorphism in the gene encoding Toll-like Receptor 3 (TLR3) is associated with sero-negative Rheumatoid Arthritis (RA) in a Danish population
    Laska M.J.
    Hansen B.
    Troldborg A.
    Lorenzen T.
    Stengaard-Pedersen K.
    Junker P.
    Nexø B.A.
    Lindegaard H.M.
    BMC Research Notes, 7 (1)
  • [32] Single Nucleotide Polymorphism Discovery of Molt Inhibiting hormone Gene 3 Exons and its Association with Growth Traits in White Shrimp (Litopenaeus vannamei)
    Li, Xi-Lian
    Xin, Jing-Jing
    Feng, Ting-Ting
    Liu, Xiao-Lin
    JOURNAL OF ANIMAL AND VETERINARY ADVANCES, 2011, 10 (21): : 2856 - 2858
  • [33] Single Nucleotide Polymorphism of CYP3A4 Intron 2 and Its Influence on CYP3A4 mRNA Expression and Liver Enzymatic Activity in Human Liver
    黄敏
    王汉明
    郭瑜
    平洁
    陈曼
    徐丹
    汪晖
    Journal of Huazhong University of Science and Technology(medical Sciences), 2015, 35 (04) : 502 - 507
  • [34] Single nucleotide polymorphism of CYP3A4 intron 2 and its influence on CYP3A4 mRNA expression and liver enzymatic activity in human liver
    Min Huang
    Han-ming Wang
    Yu Guo
    Jie Ping
    Man Chen
    Dan Xu
    Hui Wang
    Journal of Huazhong University of Science and Technology [Medical Sciences], 2015, 35 : 502 - 507
  • [35] Single nucleotide polymorphism of CYP3A4 intron 2 and its influence on CYP3A4 mRNA expression and liver enzymatic activity in human liver
    Huang, Min
    Wang, Han-ming
    Guo, Yu
    Ping, Jie
    Chen, Man
    Xu, Dan
    Wang, Hui
    JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY-MEDICAL SCIENCES, 2015, 35 (04) : 502 - 507
  • [36] Single Nucleotide Polymorphism T(861-20)C in TGFbeta1 Gene Affects its Expression in Peripheral Blood Mononuclear Cells in Russian Postmenopausal Women
    Tchetina, Elena
    Krylov, Mikhail
    Nikitinskaya, Oksana
    Demin, Nikolai
    Korotkova, Tatiana
    Toroptsova, Natalia
    Maslova, Karina
    Benevolenskaya, Lidiya
    Myakotkin, Valerii
    BONE, 2008, 43 : S45 - S45
  • [37] Differential gene expression profiles of colorectal adenocarcinomas in relation to a single nucleotide polymorphism at-25 of the 5′ untranslated region (5′UTR-25) of the RPH3AL gene
    Katkoori, Venkat R.
    Jia, Xu
    Chen, Dung-Tsa
    Grizzle, William E.
    Manne, Upender
    CANCER RESEARCH, 2006, 66 (08)
  • [38] Polymorphism of the rs1815739 Locus of the ACTN3 Gene and rs11227639 cis-eQTL Affecting Its Expression in Populations of Siberia
    B. A. Malyarchuk
    M. V. Derenko
    G. A. Denisova
    Russian Journal of Genetics, 2023, 59 : 408 - 413
  • [39] Polymorphism of the rs1815739 Locus of the ACTN3 Gene and rs11227639 cis-eQTL Affecting Its Expression in Populations of Siberia
    Malyarchuk, B. A.
    Derenko, M. V.
    Denisova, G. A.
    RUSSIAN JOURNAL OF GENETICS, 2023, 59 (04) : 408 - 413
  • [40] The 829C→T single nucleotide polymorphism in the 3′ UTR of the dihydrofolate reductase gene results in methotrexate resistance and is rare among non-Japanese American patients
    Mishra, Prasunkumar J.
    Longo, Giuseppe S. A.
    Menon, Lata G.
    Abali, Emine E.
    Humeniuk, Rita
    Cole, Peter D.
    Kamen, Barton A.
    Banerjee, Debabrata
    Bertino, Joseph R.
    CANCER RESEARCH, 2006, 66 (08)