Resistance exercise-induced changes of inflammatory gene expression within human skeletal muscle

被引:81
作者
Buford, Thomas W. [2 ]
Cooke, Matthew B. [3 ]
Willoughby, Darryn S. [1 ,3 ,4 ]
机构
[1] Baylor Univ, Dept Hlth Human Performance & Recreat, Exercise & Biochem Nutr Lab, Waco, TX 76798 USA
[2] Univ Florida, Inst Aging, Dept Aging & Geriatr Res, Div Med, Gainesville, FL USA
[3] Baylor Univ, Exercise Nutr & Resistance Training Res Unit, Waco, TX 76798 USA
[4] Baylor Univ, Baylor Biomed Sci Inst, Waco, TX 76798 USA
关键词
Inflammation; Cytokines; Resistance training; mRNA; Sarcopenia; Menopause; NECROSIS-FACTOR-ALPHA; HORMONE REPLACEMENT THERAPY; TIME-COURSE; SIGNALING PATHWAY; COX-2; PATHWAY; UNITED-STATES; STEM-CELLS; WOMEN; STRENGTH; MASS;
D O I
10.1007/s00421-009-1145-z
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Aberrant local inflammatory signaling within skeletal muscle is now considered a contributing factor to the development of sarcopenia. Recent evidence indicates that chronic resistance training contributes to the control of locally derived inflammation via adaptations to repeated, acute increases in pro-inflammatory mRNA within muscle. However, only a limited number of gene transcripts related to the inflammatory process have been examined in the literature. The present study utilized an acute bout to examine the effects of resistance exercise on several inflammatory-related genes in 24 physically active, post-menopausal women not currently undergoing hormone replacement therapy. Following a standard warm-up, participants completed a lower-body resistance exercise bout consisting of 3 sets of 10 repetitions on machine squat, leg press, and leg extension exercises (80% intensity). Muscle biopsies were obtained from the vastus lateralis of the dominant leg at baseline and 3 h following exercise. Significant (p < 0.05) up-regulation in mRNA content was observed for TNF alpha, IL1 beta, IL6, IL8, SOCS2, COX2, SAA1, SAA2, IKKB, cfos, and junB. Muscle mRNA content was not significantly altered at the 0.05 level for IL2, IL5, IL10, or IL12 (p35). Venous blood samples were also obtained at baseline as well as at 3, 24, and 48 h post-exercise. Serum was analyzed for circulating TNF alpha, IL1 beta, IL6, IL8, COX2, and SAA with no significant changes observed. These results indicate that resistance exercise is capable of up-regulating transcription of numerous inflammatory mediators within skeletal muscle, and these appear to be worthy of future examination in chronic studies.
引用
收藏
页码:463 / 471
页数:9
相关论文
共 51 条
[1]   RETRACTED: Exercise induces interleukin-8 expression in human skeletal muscle (Retracted article. See vol. 589, pg. 3407, 2011) [J].
Akerstrom, T ;
Steensberg, A ;
Keller, P ;
Keller, C ;
Penkowa, M ;
Pedersen, BK .
JOURNAL OF PHYSIOLOGY-LONDON, 2005, 563 (02) :507-516
[2]  
BADOLATO R, 1995, J IMMUNOL, V155, P4004
[3]   LEG EXTENSOR POWER AND FUNCTIONAL PERFORMANCE IN VERY OLD MEN AND WOMEN [J].
BASSEY, EJ ;
FIATARONE, MA ;
ONEILL, EF ;
KELLY, M ;
EVANS, WJ ;
LIPSITZ, LA .
CLINICAL SCIENCE, 1992, 82 (03) :321-327
[4]   Time course of molecular responses of human skeletal muscle to acute bouts of resistance exercise [J].
Bickel, CS ;
Slade, J ;
Mahoney, E ;
Haddad, F ;
Dudley, GA ;
Adams, GR .
JOURNAL OF APPLIED PHYSIOLOGY, 2005, 98 (02) :482-488
[5]   The COX-2 pathway regulates growth of atrophied muscle via multiple mechanisms [J].
Bondesen, BA ;
Mills, ST ;
Pavlath, GK .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2006, 290 (06) :C1651-C1659
[6]   The COX-2 pathway is essential during early stages of skeletal muscle regeneration [J].
Bondesen, BA ;
Mills, ST ;
Kegley, KM ;
Pavlath, GK .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2004, 287 (02) :C475-C483
[7]   Muscle strength after resistance training is inversely correlated with baseline levels of soluble tumor necrosis factor receptors in the oldest old [J].
Bruunsgaard, H ;
Bjerregaard, E ;
Schroll, M ;
Pedersen, BK .
JOURNAL OF THE AMERICAN GERIATRICS SOCIETY, 2004, 52 (02) :237-241
[8]   Endogenous interferon-γ is required for efficient skeletal muscle regeneration [J].
Cheng, Ming ;
Nguyen, Mai-Huong ;
Fantuzzi, Giamila ;
Koh, Timothy J. .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2008, 294 (05) :C1183-C1191
[9]   The molecular bases of training adaptation [J].
Coffey, Vernon G. ;
Hawley, John A. .
SPORTS MEDICINE, 2007, 37 (09) :737-763
[10]   Tumor necrosis factor α signaling in skeletal muscle:: effects of age and caloric restriction [J].
Dirks, Amie J. ;
Leeuwenburgh, Christiaan .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2006, 17 (08) :501-508