Platelet autoantibodies and lupus-associated thrombocytopenia

被引:31
作者
Michel, M
Lee, K
Piette, JC
Fromont, P
Schaeffer, A
Bierling, P
Godeau, B
机构
[1] Hop Henri Mondor, Serv Med Interne, F-94010 Creteil, France
[2] Hop Henri Mondor, EFS Ele de France, Lab Immunol Leucoplaquettaire, F-94010 Creteil, France
[3] Hop La Pitie Salpetriere, Assistance Publ Hop Paris, Serv Med Interne, Paris, France
关键词
systemic lupus erythematosus; immune thrombocytopenia; antiplatelet antibodies; MAIPA;
D O I
10.1046/j.1365-2141.2002.03817.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To characterize the antigenic targets of antiplatelet antibodies (APA) found in systemic lupus erythematosus (SLE)-associated thrombocytopenia, 48 patients with immune thrombocytopenia and SLE were compared with 20 patients with SLE who had never been thrombocytopenic. Both cases and controls were tested for circulating APA by an indirect platelet suspension immunofluorescence assay (PSIIFT) and by indirect monoclonal antibody specific immobilization of platelet antigens (MAIPA). A direct platelet suspension immunofluorescence assay (PSIFT) was also used for antibodies bound to platelets in vivo in thrombocytopenic patients; 13 of them with high titres of platelets-bound APA were investigated by direct and indirect MAIPA and platelet eluate analysis. Circulating APA were detected by PSIIFT in 88% of cases and 55% of controls (P = 0 0066) and platelet-bound antibodies were detected by PSIFT in 90% of cases. Indirect MAIPA detected specific APA (mainly directed against GpIIbIIIa) in 36% of cases and only 5% of the controls (P = 0 0076). Nine out of the 13 fully investigated thrombocytopenic patients (69%) had a positive direct MAIPA and/or APA detected in platelet eluates. In conclusion, the production of specific anti-platelet autoantibodies, mainly directed against GpIIb/IIIa, and their binding to platelet membrane plays an important role in the pathogenesis of SLE-associated thrombocytopenia.
引用
收藏
页码:354 / 358
页数:5
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