Detection of EGFR mutation of pulmonary adenocarcinoma in sputum using droplet digital PCR

被引:9
|
作者
Isaka, Tetsuya [1 ,2 ]
Yokose, Tomoyuki [3 ]
Ito, Hiroyuki [1 ]
Nakayama, Haruhiko [1 ]
Miyagi, Yohei [4 ]
Saito, Haruhiro [5 ]
Masuda, Munetaka [2 ]
机构
[1] Kanagawa Canc Ctr, Dept Thorac Surg, 2-3-2 Nakao, Yokohama, Kanagawa 2418515, Japan
[2] Yokohama City Univ, Dept Surg, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan
[3] Kanagawa Canc Ctr, Dept Pathol, 2-3-2 Nakao, Yokohama, Kanagawa 2418515, Japan
[4] Kanagawa Canc Ctr, Mol Pathol & Genet Div, Res Inst, 2-3-2 Nakao, Yokohama, Kanagawa 2418515, Japan
[5] Kanagawa Canc Ctr, Dept Thorac Oncol, 2-3-2 Nakao, Yokohama, Kanagawa 2418515, Japan
关键词
Lung cancer; Adenocarcinoma; EGFR; Digital droplet PCR; Sputum; Cytology; STAS;
D O I
10.1186/s12890-021-01468-9
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: It is still unclear whether epidermal growth factor receptor (EGFR) mutation of primary lung adenocarcinoma can be detected on sputum samples. This study aimed to examine EGFR mutations of primary lung adenocarcinoma in sputum samples using droplet digital polymerase chain reaction (ddPCR) and compare it with an EGFR mutation in surgically resected lung cancer. Methods: Sputum was prospectively collected from the patients before complete resection of the primary lung cancer at Kanagawa Cancer Center from September 2014 to May 2016. ddPCR was performed to detect EGFR exon 21 L858R point mutation (Ex21) and EGFR exon 19 deletion mutation (Ex19) in sputum samples from patients with lung adenocarcinoma. The concordance of EGFR mutation status in sputum samples and tumors in surgically resected specimen was evaluated for each positive and negative cytology group. Results: One hundred and eighteen patients with primary lung adenocarcinoma provided sputum samples. Sputum cytology was positive in 13 patients (11.0%). ddPCR detected two cases of Ex21 and two cases of Ex19 in sputum cytology positive cases. Compared to surgically resected specimens, the sensitivity, specificity, and positive predictive value of EGFR mutation (Ex19 and Ex21) detection were 80.0%, 100%, and 100%, respectively, in sputum cytology positive cases. In contrast, the sensitivity, specificity, and positive predictive value of EGFR mutation (Ex19 and Ex21) detection were 3.1%, 100%, and 100%, respectively, in sputum cytology negative cases. Conclusions: EGFR mutations in primary lung adenocarcinoma can be detected with high sensitivity in sputum samples if sputum cytology is positive.
引用
收藏
页数:11
相关论文
共 50 条
  • [1] Detection of EGFR mutation of pulmonary adenocarcinoma in sputum using droplet digital PCR
    Tetsuya Isaka
    Tomoyuki Yokose
    Hiroyuki Ito
    Haruhiko Nakayama
    Yohei Miyagi
    Haruhiro Saito
    Munetaka Masuda
    BMC Pulmonary Medicine, 21
  • [2] Detection of EGFR Activating and Resistance Mutations by Droplet Digital PCR in Sputum of EGFR-Mutated NSCLC Patients
    Hackner, Klaus
    Buder, Anna
    Hochmair, Maximilian J.
    Strieder, Matthaeus
    Grech, Christina
    Fabikan, Hannah
    Burghuber, Otto C.
    Errhalt, Peter
    Filipits, Martin
    CLINICAL MEDICINE INSIGHTS-ONCOLOGY, 2021, 15
  • [3] Comparison of EGFR Mutation Detection in Plasma Circulating Tumor DNA Using PNA Clamping and Droplet Digital PCR from Patients with Lung Adenocarcinoma
    Roh, Mee Sook
    Son, Choonhee
    MODERN PATHOLOGY, 2018, 31 : 747 - 747
  • [4] Comparison of EGFR Mutation Detection in Plasma Circulating Tumor DNA Using PNA Clamping and Droplet Digital PCR from Patients with Lung Adenocarcinoma
    Roh, Mee Sook
    Son, Choonhee
    LABORATORY INVESTIGATION, 2018, 98 : 747 - 747
  • [5] The superiority of bronchial washing fluid for detection of EGFR mutations by droplet digital PCR in lung adenocarcinoma
    Ryu, Woo Kyung
    Yong, Seung Hyun
    Lee, Sang Hoon
    Gwon, Hye Ran
    Kim, Chi Young
    Chang, Yoon Soo
    Kim, Eun Young
    RESPIROLOGY, 2023, 28 : 51 - 51
  • [6] Comparison of droplet digital PCR and conventional quantitative PCR for measuring EGFR gene mutation
    Zhang, Bo
    Xu, Chun-Wei
    Shao, Yun
    Wang, Huai-Tao
    Wu, Yong-Fang
    Song, Ye-Ying
    Li, Xiao-Bing
    Zhang, Zhe
    Wang, Wen-Jing
    Li, Li-Qiong
    Cai, Cong-Li
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2015, 9 (04) : 1383 - 1388
  • [7] Detection and quantification of EGFR T790M mutation in liquid biopsies by droplet digital PCR
    Silveira, Catarina
    Sousa, Ana Carla
    Janeiro, Andre
    Malveiro, Sara
    Teixeira, Encarnacao
    Brysch, Eva
    Pantarotto, Marcos
    Felizardo, Margarida
    Madureira, Rosa
    Nogueira, Fernando
    Guimaraes, Catia
    Matos, Cristina
    Canario, Dolores
    Bruges-Armas, Jacome
    Carmo-Fonseca, Maria
    TRANSLATIONAL LUNG CANCER RESEARCH, 2021, 10 (03) : 1200 - 1208
  • [8] Optimal method for quantitative detection of plasma EGFR T790M mutation using droplet digital PCR system
    Suzawa, Ken
    Yamamoto, Hiromasa
    Ohashi, Kadoaki
    Hashida, Shinsuke
    Tomida, Shuta
    Kubo, Toshio
    Maki, Yuho
    Soh, Junichi
    Tsukuda, Kazunori
    Kiura, Katsuyuki
    Miyoshi, Shinichiro
    Toyooka, Shinichi
    ONCOLOGY REPORTS, 2017, 37 (05) : 3100 - 3106
  • [9] Detection of the EGFR G719S Mutation in Non-small Cell Lung Cancer Using Droplet Digital PCR
    Esteva-Socias, Margalida
    Enver-Sumaya, Monica
    Gomez-Bellvert, Cristina
    Guillot, Monica
    Azkarate, Aitor
    Marse, Raquel
    Sastre, Ursula
    Blasco, Ana
    Calabuig-Farinas, Silvia
    Asensio, Victor Jose
    Terrasa, Josefa
    Obrador-Hevia, Antonia
    FRONTIERS IN MEDICINE, 2020, 7
  • [10] Droplet Digital PCR as a Molecular Tool for the Detection of the EGFR T790M Mutation in NSCLC Patients with the EGFR Activating Mutations
    Durgut, S.
    Salihefendic, L.
    Pecar, D.
    Ceko, I
    Mulahuseinovic, N.
    Izmirlija, M.
    Konjhodzic, R.
    BALKAN JOURNAL OF MEDICAL GENETICS, 2023, 26 (02) : 21 - 26