Folate-mediated targeting of albumin conjugates of paclitaxel obtained through a heterogeneous phase system

被引：43

作者：

Dosio, Franco ^{[1
]}

Arpicco, Silvia ^{[1
]}

Stella, Barbara ^{[1
]}

Brusa, Paola ^{[1
]}

Cattel, Luigi ^{[1
]}

机构：

[1] Univ Torino, Dipartimento Sci & Tecnol Farmaco, I-10125 Turin, Italy

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2009年 / 382卷 / 1-2期

关键词：

Folate receptor; Targeted drug delivery; Paclitaxel; Macromolecular conjugates; Cibacron Blue; IN-VIVO; DRUG-DELIVERY; RECEPTOR; PRODRUGS; VITRO; FORMULATIONS; IMMUNOTOXINS; ENDOCYTOSIS; MECHANISM; ANTIBODY;

D O I：

10.1016/j.ijpharm.2009.08.018

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The study developed cytotoxic macromolecular conjugates that specifically target the folate receptor and deliver the drug into cell cytoplasm. The anticancer agent paclitaxel was conjugated to human serum albumin (HSA) and this drug-albumin conjugate was further equipped with folic acid, linked via an extended poly(ethylene glycol) spacer. Preparation was carried out in a heterogeneous phase system exploiting the binding ability of Cibacron Blue dye to HSA. Unreacted reagents were easily removed and, after purification by gel filtration, the conjugate was fully characterized. Binding and in vitro cytotoxicity studies on human nasopharyngeal epidermal carcinoma KB and colorectal carcinoma HT-29 cells (as negative control) demonstrated increased selectivity and anti-tumoral activity. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：117 / 123

页数：7

共 36 条

[1] Folate-mediated targeting of polymeric conjugates of gemcitabine [J].

Cavallaro, G ;

Mariano, L ;

Salmaso, S ;

Caliceti, P ;

Gaetano, G .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 307 (02) :258-269

[2] Biological and clinical characterization of paclitaxel poliglumex (PPX, CT-2103), a macromolecular polymer-drug conjugate [J].

Chipman, Stewart D. ;

Oldham, Fred B. ;

Pezzoni, Gabriella ;

Singer, Jack W. .

INTERNATIONAL JOURNAL OF NANOMEDICINE, 2006, 1 (04) :375-383

[3] Dye-ligand affinity systems [J].

Denizli, A ;

Piskin, E .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2001, 49 (1-3) :391-416

[4] A NEW APPROACH IN THE SYNTHESIS OF IMMUNOTOXINS - RIBOSOME-INACTIVATING PROTEIN NONCOVALENTLY BOUND TO MONOCLONAL-ANTIBODY [J].

DOSIO, F ;

BRUSA, P ;

DELPRINO, L ;

GROSA, G ;

CERUTI, M ;

CATTEL, L .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1994, 83 (02) :206-211

[5]

DOSIO F, 1993, FARMACO, V48, P105

[6] Preparation, characterization and properties in vitro and in vivo of a paclitaxel-albumin conjugate [J].

Dosio, F ;

Brusa, P ;

Crosasso, P ;

Arpicco, S ;

Cattel, L .

JOURNAL OF CONTROLLED RELEASE, 1997, 47 (03) :293-304

[7] Poly(ethylene glycol)-human serum albumin-paclitaxel conjugates: preparation, characterization and pharmacokinetics [J].

Dosio, F ;

Arpicco, S ;

Brusa, P ;

Stella, B ;

Cattel, L .

JOURNAL OF CONTROLLED RELEASE, 2001, 76 (1-2) :107-117

[8] Phase 1 and pharmacokinetic study of weekly docosahexaenoic acid-paclitaxel, TaxoprexinA®, in resistant solid tumor malignancies [J].

Fracasso, Paula M. ;

Picus, Joel ;

Wildi, Jonathan D. ;

Goodner, Sherry A. ;

Creekmore, Allison N. ;

Gao, Feng ;

Govindan, Ramaswamy ;

Ellis, Matthew J. ;

Tan, Benjamin R. ;

Linette, Gerald P. ;

Fu, ChauHwei J. ;

Pentikis, Helen S. ;

Zumbrun, Stephen C. ;

Egorin, Merrill J. ;

Bellet, Robert E. .

CANCER CHEMOTHERAPY AND PHARMACOLOGY, 2009, 63 (03) :451-458

[9] Targeting folate receptor with folate linked to extremities of poly(ethylene glycol)-grafted liposomes: In vitro studies [J].

Gabizon, A ;

Horowitz, AT ;

Goren, D ;

Tzemach, D ;

Mandelbaum-Shavit, F ;

Qazen, MM ;

Zalipsky, S .

BIOCONJUGATE CHEMISTRY, 1999, 10 (02) :289-298

[10] CHROMATOGRAPHY OF PLASMA-PROTEINS ON IMMOBILIZED CIBACRON BLUE F3GA - MECHANISM OF THE MOLECULAR INTERACTION [J].

GIANAZZA, E ;

ARNAUD, P .

BIOCHEMICAL JOURNAL, 1982, 203 (03) :637-641

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