Complicated genotypes circulating among treatment naive HIV-1 patients in Guangzhou, China

被引：14
作者：
Lan, Yun [1 ]
He, Xiang [2 ]
Li, Linghua [1 ]
Zhou, Pingping [2 ]
Huang, Xuhe [2 ]
Deng, Xizi [1 ]
Li, Junbin [1 ]
Fan, Qinghong [1 ]
Li, Feng [1 ]
Tang, Xiaoping [1 ]
Cai, Weiping [1 ]
Hu, Fengyu [1 ]
机构：
[1] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, 627 Dongfeng East Rd, Guangzhou 510030, Peoples R China
[2] Guangdong Prov Ctr Dis Control & Prevent, Guangdong Prov Inst Publ Hlth, 160 Qunxian Rd, Guangzhou 511430, Peoples R China
来源：
INFECTION GENETICS AND EVOLUTION | 2021年 / 87卷
关键词：
HIV-1; Genotype; Risk factor; Guangzhou; China; DRUG-RESISTANCE; SEQUENCES; DIVERSITY; EPIDEMIC; STRAIN; MEN; ART; SEX;
D O I：
10.1016/j.meegid.2020.104673
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Guangzhou city is the political, economic, and cultural center of the Guangdong Province, China. The molecular epidemiological characteristics of HIV-1 in Guangzhou are not widely known. The aim of this study was to explore the characteristics of HIV-1 genotypes among treatment naive HIV/AIDS patients living in Guangzhou. HIV-1 RNA was extracted from serum specimens. The partial pol gene of the HIV-1 genome was amplified and sequenced. The genotypes were screened using the subtyping tool COMET and further confirmed by phylogenetic analysis, with the exception of the URFs that were analyzed by jpHMM and RIP. The distributions of HIV genotypes in different risk populations were analyzed. Subsequently, pol sequences were used to construct transmission networks and analyze drug resistance. Twelve HIV-1 genotypes including 3 subtypes and 9 CRFs, with several URFs were identified from 1388 HIV-1 sequences, which were derived from 1490 patients. The main genotypes circulating in Guangzhou were CRF07_BC (38.3%), CRF01_AE (32.3%), and CRF55_01B (10.7%). CRF01_AE was the secondary dominant strain and multiple lineages of CRF01_AE had been identified in Guangzhou. The 01B recombinant forms, including CRF55_01B, CRF59_01B and CRF68_01B, have circulated widely in Guangzhou. 42.22% (586/1388) of the study sequences fell into 143 transmission networks, and the three main clusters revealed that sequences from MSM and HET populations were intermixed. 5.40% (75/1388) of patients had pre-treatment drug resistance. The HIV-1 strains that were present in Guangzhou have demonstrated complex genotypes. Particular attention should be given on these genotypes for the further strategy of prevention and intervention of HIV transmission.\
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页数：9
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