The Sensitive and Selective Optical Detection of Mercury(II) Ions by Using a Phosphorothioate DNAzyme Strategy

被引：41

作者：

Zhang, Dan

Deng, Minggang

Xu, Liang

Zhou, Yangyang

Yuwen, Jing

Zhou, Xiang ^{[1
,2
]}

机构：

[1] Wuhan Univ, Coll Chem & Mol Sci, Key Lab Biomed Polymers, Minist Educ, Wuhan 430072, Peoples R China

[2] Beijing Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 33期

关键词：

mecury ions; optical detection; phosphorothioate DNAzyme; sensors; TELOMERIC G-QUADRUPLEX; COLORIMETRIC DETECTION; PEROXIDASE-ACTIVITY; CATALYTIC BEACONS; HG2+ IONS; IN-VITRO; TURN-ON; DNA; SENSOR; COMPLEXES;

D O I：

10.1002/chem.200901268

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A study was conducted to develop a new approach to form G-quadruplex complexes efficiently by modifying the sequence. The study also investigated the use of these conformations as functional probes. The investigations were conducted using a phosphorothioate DNAzyme strategy. The phosphorothioate DNAzyme strategy was used to perform sensitive and selective optical detection of mercury(II) ions. The utilization of the DNAzyme operated by the phosphorothioate G-quadruplex formed from T4G4-S 3 combined the advantage of sensitive and selective optical detection of mercury(II) ions. The modification of oligonuleotides with sulfur and the regulation of the phosphorothioate G-quadruplex by metal ions such as Hg 2+ were also combined to develop the strategy for sensitive and selective optical detection of mercury(II) ions.

引用

页码：8117 / 8120

页数：4