Betulinic acid inhibits IL-1β-induced inflammation by activating PPAR-γ in human osteoarthritis chondrocytes

被引：47
作者：
Wang Jingbo [1 ]
Chen Aimin [1 ]
Wu Qi [1 ]
Li Xin [1 ]
Li Huaining [2 ]
机构：
[1] Childrens Hosp Harbin, Dept Orthoped, Harbin 150010, Peoples R China
[2] Childrens Hosp Harbin, Dept Cardiovasc Surg, Harbin 150010, Peoples R China
来源：
INTERNATIONAL IMMUNOPHARMACOLOGY | 2015年 / 29卷 / 02期
关键词：
Betulinic acid; Osteoarthritis chondrocyte; IL-1; beta; NF-kappa B; PPAR-gamma; NF-KAPPA-B; ARTICULAR-CARTILAGE; PATHOGENESIS; EXPRESSION; MANAGEMENT; RECEPTORS; MECHANISM; CYTOKINES; DISEASE;
D O I：
10.1016/j.intimp.2015.09.009
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Betulinic acid (BA), a triterpenoid isolated from birch bark, has been reported to have anti-inflammatory effects. In this study, we investigated the anti-osteoarthritic effects of BA in IL-1 beta-stimulated human osteoarthritis chondrocytes. Human osteoarthritis chondrocytes were pre-incubated with BA (6, 12, 24 mu M) for 12 h and then treated with IL-1 beta (10 ng/ml). The production of PGE2 and NO were detected by ELISA and Griess reagent. The expression of NF-kappa B, I kappa B, and PPAR-gamma were detected by Western blotting. The results showed that BA dose-dependently inhibited IL-1 beta-induced MMP-1, MMP-3, MMP-13, PGE2 and NO productions. BA also inhibited IL-1 beta-induced NF-kappa B activation. Furthermore, BA was found to activate PPAR-gamma and the inhibition of PGE2 and NO by BA can be reversed by PPAR-gamma antagonist GW9662. In conclusion, these results suggested that BA inhibited IL-1 beta-induced inflammation in osteoarthritis chondrocytes by activating PPAR-gamma. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：687 / 692
页数：6
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