Rapid detection of silkworm microsporidia by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

被引：6

作者：

Kajiwara, Hideyuki ^{[1
]}

Murakami, Ritsuko ^{[2
]}

机构：

[1] Natl Agr & Food Res Org NARO, Adv Anal Ctr, Tsukuba, Ibaraki 3058612, Japan

[2] NARO, Inst Agrobiol Sci, Tsukuba, Ibaraki 3058634, Japan

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 2019年 / 166卷

关键词：

Bombyx mori; MALDI biotyping; Matrix-assisted laser desorption/ionization; time-of-flight mass spectrometry (MALDI-TOFMS); Microsporidia; Pebrine; NOSEMA-BOMBYCIS; IDENTIFICATION; AMPLIFICATION; PATHOGENS; ASSAY;

D O I：

10.1016/j.jip.2019.107223

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Microsporidia cause the disease pebrine in silkworm and are known to be detrimental to sericulture and beekeeping. The microsporidian species Nosema bombycis was rapidly identified in silkworm (Bombyx mori) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Four types of microsporidian spores purified from infected silkworm could be distinguished based on the differences in their mass fingerprints. Microsporidia growing in a silkworm larva were also identified based on their mass spectra after rapid separation using filtration and centrifugation for 30 min.

引用

页数：4

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