Molecular identification and morphological description of Micropogonias megalops, Cynoscion othonopterus, C. reticulatus and Menticirrhus nasus larvae, collected in the upper Gulf of California during Summer 2012

被引：4

作者：

Camacho-Gastelum, Rosabel ^{[1
]}

Diaz-Viloria, Noe ^{[1
]}

Sanchez-Velasco, Laura ^{[1
]}

Jimenez-Rosenberg, Sylvia P. A. ^{[1
]}

Perez-Enriquez, Ricardo ^{[2
]}

机构：

[1] Inst Politecn Nacl, Ctr Interdisciplinario Ciencias Marinas, La Paz, Mexico

[2] Ctr Invest Biol Noroeste, La Paz, Mexico

来源：

MITOCHONDRIAL DNA PART A | 2017年 / 28卷 / 03期

关键词：

Cytochrome c oxidase; subunit; 1; molecular markers; molecular taxonomy; Sciaenidae larvae; DNA; PERCIFORMES; PCR;

D O I：

10.3109/19401736.2015.1136302

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Sciaenidae fish larvae were collected from the upper Gulf of California during September 2012 using a conical net (505 mm) through surface tows. These were pre-classified into four larval morphotypes, based on external characteristics (mainly meristic and pigmentation). Partial sequences of cytochrome c oxidase, subunit 1 and 16S rRNA (16S) genes of mitochondrial DNA, were used in molecular genetic identification from each larval morphotype. Genetic results indicated the identification of four larval morphotypes as Micropogonias megalops, Cynoscion othonopterus, C. reticulatus and Menticirrhus nasus. Pigmentation patterns of larvae described after molecular genetic identification made it possible to distinguish between M. megalops, M. nasus and C. othonopterus (postflexion). However, pigmentation was not reliable for differentiating between preflexion larvae of C. othonopterus and C. reticulatus. From these results, both morphological and genetic approaches were proposed as complementary tools in taxonomic studies of ichthyo-plankton, particularly in early fish larvae identification of congeneric species with similar morphological characteristics.

引用

页码：416 / 423

页数：8

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