Overexpression and RNA interference of Ap-cyclic AMP-response element binding protein-2, a repressor of long-term facilitation, in Aplysia kurodai sensory-to-motor synapses

被引：20

作者：

Lee, JA ^{[1
]}

Kim, H ^{[1
]}

Lee, YS ^{[1
]}

Kaang, BK ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Inst Mol Biol & Genet,Natl Res Lab, Seoul 151742, South Korea

来源：

NEUROSCIENCE LETTERS | 2003年 / 337卷 / 01期

关键词：

Aplysia; cyclic AMP-response element binding protein-2; RNA interference; long-term facilitation; transcription factor; sensory-to-motor synapse;

D O I：

10.1016/S0304-3940(02)01285-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

cyclic AMP-response element binding protein-2 (CREB2) is a member of the CREB/transcription factor (CREB/ATF4) family. CREB2 is a transcription factor known to be involved in Aplysia long-term facilitation. To further examine the role of ApCREB2 on long-term synaptic facilitation, we isolated ApCREB2 from Aplysia kurodai in full-length cDNA library, and found that the overexpression of ApCREB2 blocked 5-hydroxytryptamine (5-HT)-induced long-term synaptic facilitation in Aplysia sensory-to-motor synapses. Furthermore, a single pulse of 5-HT, which normally induces only short-term facilitation, in the presence of ApCREB2 inhibition by RNA interference, induced long-term facilitation in Aplysia sensory-to-motor synapses. These results suggest that ApCREB2 is a functional repressor of long-term facilitation in Aplysia sensory-to-motor synapses. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：9 / 12

页数：4

共 11 条

[1] Positive and negative regulatory mechanisms that mediate long-term memory storage [J].

Abel, T ;

Kandel, E .

BRAIN RESEARCH REVIEWS, 1998, 26 (2-3) :360-378

[2] APLYSIA CREB2 REPRESSES LONG-TERM FACILITATION - RELIEF OF REPRESSION CONVERTS TRANSIENT FACILITATION INTO LONG-TERM FUNCTIONAL AND STRUCTURAL-CHANGE [J].

BARTSCH, D ;

GHIRARDI, M ;

SKEHEL, PA ;

KARL, KA ;

HERDER, SP ;

CHEN, M ;

BAILEY, CH ;

KANDEL, ER .

CELL, 1995, 83 (06) :979-992

[3] CREB1 encodes a nuclear activator, a repressor, and a cytoplasmic modulator that form a regulatory unit critical for long-term facilitation [J].

Bartsch, D ;

Casadio, A ;

Karl, KA ;

Serodio, P ;

Kandel, ER .

CELL, 1998, 95 (02) :211-223

[4] INJECTION OF THE CAMP-RESPONSIVE ELEMENT INTO THE NUCLEUS OF APLYSIA SENSORY NEURONS BLOCKS LONG-TERM FACILITATION [J].

DASH, PK ;

HOCHNER, B ;

KANDEL, ER .

NATURE, 1990, 345 (6277) :718-721

[5] Genetic dissection of structural and functional components of synaptic plasticity .3. CREB is necessary for presynaptic functional plasticity [J].

Davis, GW ;

Schuster, CM ;

Goodman, CS .

NEURON, 1996, 17 (04) :669-679

[6]

IMPEY S, 1996, NEURON, V16, P73

[7] ACTIVATION OF CAMP-RESPONSIVE GENES BY STIMULI THAT PRODUCE LONG-TERM FACILITATION IN APLYSIA SENSORY NEURONS [J].

KAANG, BK ;

KANDEL, ER ;

GRANT, SGN .

NEURON, 1993, 10 (03) :427-435

[8] Overexpression of and RNA interference with the CCAAT enhancer-binding protein on long-term facilitation of Aplysia sensory to motor synapses [J].

Lee, JA ;

Kim, HK ;

Kim, KH ;

Han, JH ;

Lee, YS ;

Lim, CS ;

Chang, DJ ;

Kubo, T ;

Kaang, BK .

LEARNING & MEMORY, 2001, 8 (04) :220-226

[9] A CRITICAL PERIOD FOR MACROMOLECULAR-SYNTHESIS IN LONG-TERM HETEROSYNAPTIC FACILITATION IN APLYSIA [J].

MONTAROLO, PG ;

GOELET, P ;

CASTELLUCCI, VF ;

MORGAN, J ;

KANDEL, ER ;

SCHACHER, S .

SCIENCE, 1986, 234 (4781) :1249-1254

[10] REQUIREMENT OF A CRITICAL PERIOD OF TRANSCRIPTION FOR INDUCTION OF A LATE-PHASE OF LTP [J].

NGUYEN, PV ;

ABEL, T ;

KANDEL, ER .

SCIENCE, 1994, 265 (5175) :1104-1107

← 1 2 →