Disaccharide phosphorylases: Structure, catalytic mechanisms and directed evolution

被引：19

作者：

Sun, Shangshang ^{[1
]}

You, Chun ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, 32 West 7th Ave, Tianjin 300308, Peoples R China

[2] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

[3] Natl Technol Innovat Ctr Synthet Biol, Tianjin 300308, Peoples R China

来源：

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY | 2021年 / 6卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Disaccharide phosphorylase; Classification; Structure and domains; Substrate specificity; Enzyme engineering; N-BIOSE-I; SUCROSE PHOSPHORYLASE; CELLOBIOSE PHOSPHORYLASE; MALTOSE PHOSPHORYLASE; TREHALOSE PHOSPHORYLASE; SUBSTRATE RECOGNITION; ACCEPTOR SPECIFICITY; CRYSTAL-STRUCTURE; CLOSTRIDIUM-THERMOCELLUM; PRACTICAL PREPARATION;

D O I：

10.1016/j.synbio.2021.01.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding potential for the bio-catalytic conversion of common table sugar into products with attractive properties. They are modular enzymes that form active homo-oligomers. From a mechanistic as well as a structural point of view, they are similar to glycoside hydrolases or glycosyltransferases. As the majority of DSPs show strict stereo- and regiospecificities, these enzymes were used to synthesize specific disaccharides. Currently, protein engineering of DSPs is pursued in different laboratories to broaden the donor and acceptor substrate specificities or improve the industrial particularity of naturally existing enzymes, to eventually generate a toolbox of new catalysts for glycoside synthesis. Herein we review the characteristics and classifications of reported DSPs and the glycoside products that they have been used to synthesize.

引用

页码：23 / 31

页数：9

共 92 条

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