Preservation of ancestral Cretaceous microflora recovered from a hypersaline oil reservoir

被引：12

作者：

Gales, Gregoire ^{[1
,2
]}

Tsesmetzis, Nicolas ^{[3
]}

Neria, Isabel ^{[2
]}

Alazard, Didier ^{[2
]}

Coulon, Stephanie ^{[4
]}

Lomans, Bart P. ^{[5
]}

Morin, Dominique ^{[4
]}

Ollivier, Bernard ^{[2
]}

Borgomano, Jean ^{[1
]}

Joulian, Catherine ^{[4
]}

机构：

[1] Aix Marseille Univ, Ctr St Charles, CEREGE, Case 67,3 Pl Victor Hugo, F-13331 Marseille, France

[2] Univ Sud Toulon Var, Aix Marseille Univ, CNRS, INSU,IRD,MIO,UM 110, F-13288 Marseille 09, France

[3] Shell Int Explorat & Prod Inc, 3333 Highway 6 South, Houston, TX 77082 USA

[4] Bur Rech Geol & Minieres, Unite BioGeochim Environm, 3 Ave Claude Guillemin,BP 36009, F-45060 Orleans 2, France

[5] Shell Global Solut Int BV, Emerging Technol Subsurface Projects & Technol, Kessler Pk 1, NL-2288 GS Rijswijk, Netherlands

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

SP-NOV; GEN; NOV; CRUDE-OIL; HALOPHILIC ARCHAEON; EMENDED DESCRIPTION; DEEP SUBSURFACE; RED-SEA; DNA; DIVERSITY; BACTERIUM;

D O I：

10.1038/srep22960

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Microbiology of a hypersaline oil reservoir located in Central Africa was investigated with molecular and culture methods applied to preserved core samples. Here we show that the community structure was partially acquired during sedimentation, as many prokaryotic 16S rRNA gene sequences retrieved from the extracted DNA are phylogenetically related to actual Archaea inhabiting surface evaporitic environments, similar to the Cretaceous sediment paleoenvironment. Results are discussed in term of microorganisms and/or DNA preservation in such hypersaline and Mg-rich solutions. High salt concentrations together with anaerobic conditions could have preserved microbial/molecular diversity originating from the ancient sediment basin wherein organic matter was deposited.

引用

页数：10

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