An artificial protein with the biological activity of the differentiation factor for the HL-60 cell line of human promyelocyte leukemia

被引：2

作者：

Chertkova, RV ^{[1
]}

Kostanyan, IA ^{[1
]}

Astapova, MV ^{[1
]}

Surina, EA ^{[1
]}

Dolgikh, DA ^{[1
]}

Kirpichnikov, MP ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

来源：

RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY | 2003年 / 29卷 / 01期

关键词：

albebetin; artificial proteins; HLDF-6; peptide; HLDF differentiation factor; HL-60 cell line;

D O I：

10.1023/A:1022274301659

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ABB-df artificial protein was prepared by inserting the TGENHR biologically active peptide corresponding to the 41-46 sequence of the differentiation factor for the HL-60 cell line of the human promyelocyte leukemia into the N-terminus of the polypeptide chain of albebetin, an artificial protein with the preset structure. The ABB-df protein was found to induce the differentiation of HL-60 cells and to inhibit their proliferation; its efficiency was almost the same as that of the starting peptide. According to CD spectroscopy, the inclusion of the peptide fragment into albebetin exerts virtually no effect on the regular secondary structure of albebetin.

引用

页码：26 / 32

页数：7

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