Effects of inoculum size and incubation time on broth microdilution susceptibility testing of lactic acid bacteria

被引:31
作者
Egervarn, Maria
Lindmark, Hans
Roos, Stefan
Huys, Geert
Lindgren, Sven
机构
[1] Natl Food Adm Toxicol Lab, Div Microbiol, SE-75126 Uppsala, Sweden
[2] Swedish Univ Agr Sci, Dept Microbiol, S-75007 Uppsala, Sweden
[3] Univ Ghent, Microbiol Lab, B-9000 Ghent, Belgium
关键词
D O I
10.1128/AAC.00637-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Inoculum size and incubation time were varied during broth microdilution testing of the susceptibilities of 35 strains of lactic acid bacteria to six antibiotics. An increase in either parameter resulted in elevated MICs for all species. An inoculum of 3 x 10(5) CFU/ml is recommended to assess the antibiotic susceptibilities of these bacteria by using broth microdilution.
引用
收藏
页码:394 / 396
页数:3
相关论文
共 22 条
[1]  
[Anonymous], 2006, METH DIL ANT SUSC TE
[2]  
[Anonymous], PROKARYOTES EVOLVING
[3]  
BOERNER J, 1995, ZBL BAKT-INT J MED M, V283, P49
[4]  
*CLIN LAB STAND I, 2005, PUBL CLIN LAB STAND
[5]   HIGH-LEVELS OF SPONTANEOUS DRUG-RESISTANCE IN LACTOBACILLUS [J].
CURRAGH, HJ ;
COLLINS, MA .
JOURNAL OF APPLIED BACTERIOLOGY, 1992, 73 (01) :31-36
[6]   Antibiotic susceptibility of Lactobacillus and Bifidobacterium species from the human gastrointestinal tract [J].
Delgado, S ;
Flórez, AB ;
Mayo, B .
CURRENT MICROBIOLOGY, 2005, 50 (04) :202-207
[7]   Antimicrobial susceptibilities of Lactococcus lactis and Lactococcus garvieae and a proposed method to discriminate between them [J].
Elliott, JA ;
Facklam, RR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (05) :1296-1298
[8]   Antimicrobial susceptibility of lactic acid bacteria isolated from a cheese environment [J].
Flórez, AB ;
Delgado, S ;
Mayo, B .
CANADIAN JOURNAL OF MICROBIOLOGY, 2005, 51 (01) :51-58
[9]   Influence of variations in test methods on susceptibility of Haemophilus influenzae to ampicillin, azithromycin, clarithromycin, and telithromycin [J].
Fuchs, PC ;
Barry, AL ;
Brown, SD .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :43-46
[10]   RECOVERY OF VANCOMYCIN-RESISTANT GRAM-POSITIVE COCCI FROM CHILDREN [J].
GREEN, M ;
WADOWSKY, RM ;
BARBADORA, K .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :484-488