High consistency enzymatic hydrolysis of hardwood substrates

被引:98
作者
Zhang, Xiao [1 ]
Qin, Wenjuan [2 ]
Paice, Michael G. [1 ]
Saddler, John N. [2 ]
机构
[1] FPInnovat Paprican Div PAPRICAN, Pointe Claire, PQ H9R 3J9, Canada
[2] Univ British Columbia, Fac Forestry, Vancouver, BC V6T 1Z4, Canada
关键词
High consistency hydrolysis; Lignocellulosic substrates; Fermentation; Ethanol; Rheological problem; STEAM-PRETREATED SOFTWOOD; ETHANOL-PRODUCTION; FERMENTATION; INHIBITION; BIOMASS; CELLULASES; YIELDS;
D O I
10.1016/j.biortech.2009.06.082
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
The feasibility of using a laboratory peg mixer to carry out high consistency enzymatic hydrolysis of lignocellulosic substrates was investigated. Two hardwood substrates, unbleached hardwood pulp (UBHW) and organosolv pretreated poplar (OPP), were used in this study. Hydrolysis of UBHW and OPP at 20% substrate consistency led to a high glucose concentration in the final hydrolysate. For example, a 48 h enzymatic hydrolysis of OPP resulted in a hydrolysate with 158 g/L of glucose. This is the highest glucose concentration ever obtained from enzymatic hydrolysis of lignocellulosic substrates. Fermentation of UBHW and OPP hydrolysates with high glucose content led to high ethanol concentrations, 50.4 and 63.1 g/L, respectively after fermentation. Our results demonstrate that using common pulping equipment to carry out high consistency hydrolysis can overcome the theological problems and greatly increase the sugar and ethanol concentrations after the hydrolysis and fermentation. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:5890 / 5897
页数:8
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