Pharmacological analysis of the neutrophil migration induced by D. rostrata lectin: Involvement of cytokines and nitric oxide

被引：29

作者：

Figueiredo, Jozi G. ^{[2
]}

Bitencourt, Flavio S. ^{[1
]}

Mota, Mario R. L. ^{[1
]}

Silvestre, Priscila P. ^{[1
]}

Aguiar, Cibele N. ^{[1
]}

Benevides, Raquel G. ^{[2
]}

Nascimento, Kyria S. ^{[2
]}

de Moura, Tales R. ^{[2
]}

Dal-Secco, Daniela ^{[4
]}

Assreuy, Ana M. S. ^{[3
]}

Cunha, Fernando de Q. ^{[4
]}

Vale, Marcus R. ^{[1
]}

Cavada, Benildo S. ^{[2
]}

Alencar, Nylane M. N. ^{[1
]}

机构：

[1] Univ Fed Ceara, Dept Fisiol & Farmacol, BR-60430270 Fortaleza, CE, Brazil

[2] Univ Fed Ceara, Dept Bioquim & Biol Mol, BR-60451970 Fortaleza, CE, Brazil

[3] Univ Estadual Ceara, Inst Super Ciencias Biomed, BR-60740903 Fortaleza, CE, Brazil

[4] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Farmacol, Ribeirao Preto, SP, Brazil

来源：

TOXICON | 2009年 / 54卷 / 06期

关键词：

Cytokines; Dioclea rostrata; Inflammation; Lectin; Leukocyte migration; Nitric oxide; Macrophage; IN-VIVO; BINDING LECTIN; LYMPHOCYTE-ACTIVATION; CHEMOTACTIC CYTOKINES; LEUKOCYTE MIGRATION; PLANT-LECTINS; PAW EDEMA; SEEDS; MACROPHAGES; AGGLUTININ;

D O I：

10.1016/j.toxicon.2009.05.037

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

In the present study, we investigated the involvement of resident cell and inflammatory mediators in the neutrophil migration induced by chemotactic activity of a glucose/mannose-specific lectin isolated from Dioclea rostrata seeds (DrosL). Rats were injected i.p. with DrosL (125-1000 mu g/cavity), and at 2-96 h thereafter the leukocyte counts in peritoneal fluid were determined. DrosL-induced a dose-dependent neutrophil migration accumulation, which reached maximal response at 24 h after injection and declines thereafter. The carbohydrate ligand nearly abolished the neutrophil influx. Pre-treatment of peritoneal cavities with thioglycolate which increases peritoneal macrophage numbers, enhanced neutrophil migration induced by DrosL by 303%. However, the reduction of peritoneal mast cell numbers by treatment of the cavities with compound 48/80 did not modify DrosL-induced neutrophil migration. The injection into peritoneal cavities of supernatants from macrophage cultures stimulated with DrosL (125, 250 and 500 mu g/ml) induced neutrophil migration. In addition, DrosL treatment induced cytokines (TNF-alpha, IL-1 beta and CINC-1) and NO release into the peritoneal cavity of rats. Finally, neutrophil chemotaxis assay in vitro showed that the lectin (15 and 31 mu g/ml) induced neutrophil chemotaxis by even 180%. In conclusion, neutrophil migration induced by D. rostrata lectin occurs by way of the release of NO and cytokines such as IL-1 beta, TNF-alpha and CINC-1. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：736 / 744

页数：9

共 68 条

[1]

ADAMS DO, 1984, ANNU REV IMMUNOL, V2, P283, DOI 10.1146/annurev.iy.02.040184.001435

[2] Nitric oxide synthases: structure, function and inhibition [J].

Alderton, WK ;

Cooper, CE ;

Knowles, RG .

BIOCHEMICAL JOURNAL, 2001, 357 (03) :593-615

[3] Leguminous lectins as tools for studying the role of sugar residues in leukocyte recruitment [J].

Alencar, NMN ;

Teixeira, EH ;

Assreuy, AMS ;

Cavada, BS ;

Flores, CA ;

Ribeiro, RA .

MEDIATORS OF INFLAMMATION, 1999, 8 (02) :107-113

[4] Anti-inflammatory and antimicrobial effect of lectin from Lonchocarpus sericeus seeds in an experimental rat model of infectious peritonitis [J].

Alencar, NMN ;

Cavalcante, CF ;

Vasconcelos, MP ;

Leite, KB ;

Aragao, KS ;

Assreuy, AMS ;

Nogueira, NAP ;

Cavada, BS ;

Vale, MR .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2005, 57 (07) :919-922

[5] Vatairea macrocarpa lectin induces paw edema with leukocyte infiltration [J].

Alencar, NMN ;

Assreuy, AMS ;

Criddle, DN ;

Souza, EP ;

Soares, PMG ;

Havt, A ;

Aragao, KS ;

Bezerra, DP ;

Ribeiro, RA ;

Cavada, BS .

PROTEIN AND PEPTIDE LETTERS, 2004, 11 (02) :195-200

[6] The galactose-binding lectin from Vatairea macrocarpa seeds induces in vivo neutrophil migration by indirect mechanism [J].

Alencar, NMN ;

Assreuy, AMS ;

Alencar, VBM ;

Melo, SC ;

Ramos, MV ;

Cavada, BS ;

Cunha, FQ ;

Ribeiro, RA .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2003, 35 (12) :1674-1681

[7]

Alencar NMN, 2007, N-S ARCH PHARMACOL, V374, P275, DOI 10.1007/s00210-006-0124-8

[8] Helianthus tuberosus agglutinin directly induces neutrophil migration, which can be modulated/inhibited by resident mast cells [J].

Alencar, VBM ;

Brito, GAC ;

Alencar, NMN ;

Assreuy, AMS ;

Pinto, VPT ;

Teixeira, EH ;

Souza, EP ;

Debray, H ;

Ribeiro, RA ;

Cavada, BS .

BIOCHEMISTRY AND CELL BIOLOGY, 2005, 83 (05) :659-666

[9] Pro-inflammatory effect of Arum maculatum lectin and role of resident cells [J].

Alencar, VBM ;

Alencar, NMN ;

Assreuy, AMS ;

Mota, ML ;

Brito, GAC ;

Aragao, KS ;

Bittencourt, FS ;

Pinto, VPT ;

Debray, H ;

Ribeiro, RA ;

Cavada, BS .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2005, 37 (09) :1805-1814

[10] Lectin of Pisum arvense seeds induces in-vivo and in-vitro neutrophil migration [J].

Alencar, VBM ;

Assreuy, AMS ;

Alencar, NMN ;

Meireles, AVP ;

Mota, MRL ;

Aragao, KS ;

Cajazeiras, JB ;

Nagano, CS ;

Brito, GAC ;

Silva, LIMM ;

Pinto, VPT ;

Sampaio, AH ;

Debray, H ;

Cavada, BS ;

Ribeiro, RA .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2005, 57 (03) :375-381

← 1 2 3 4 5 6 7 →