Spectroscopic Studies on the Binding of a New Quinolone Antibacterial Agent: Sinafloxacin to DNA

被引：64
作者：
Fei, Yan [1 ,2 ,3 ]
Lu, Guocai [4 ]
Fan, Guorong [1 ,2 ]
Wu, Yutian [1 ,2 ]
机构：
[1] Second Mil Med Univ, Dept Pharmaceut Anal, Sch Pharm, Shanghai 200433, Peoples R China
[2] Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China
[3] 175th Hosp PLA, Dept Pharm, Zhangzhou 363000, Peoples R China
[4] Second Mil Med Univ, Inst Basic Med Sci, Ctr New Drug Evaluat, Shanghai 200433, Peoples R China
来源：
ANALYTICAL SCIENCES | 2009年 / 25卷 / 11期
关键词：
BOVINE SERUM-ALBUMIN; FLUORESCENT-PROBE; NUCLEIC-ACIDS; COMPLEXES; MECHANISM; BLUE;
D O I：
10.2116/analsci.25.1333
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The binding of sinafloxacin to DNA has been studied by fluorescence spectroscopy and UV-vis absorption spectroscopy. The variations in the spectroscopic characteristics of sinafloxacin upon addition of DNA were observed. Salt concentration effect, KI quenching, fluorescence polarization, DNA denaturation experiments, melting temperature (T-m) curves and viscosity measurements were studied to investigate the binding mechanism. It was proved that the fluorescence quenching of sinafloxacin is a result of the formation of sinafloxacin-DNA complex. Quenching constants were determined using the Stern-Volmer equation. From the experiment evidence, the major binding mode of sinafloxacin with DNA was evaluated to be the groove binding.
引用
收藏
页码：1333 / 1338
页数：6
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