Laboratory diagnosis of a new outbreak of acute African swine fever in smallholder pig farms in Jos, Nigeria

被引：3

作者：

Tizhe, Emmanuel V. ^{[1
]}

Luka, Pam D. ^{[2
]}

Adedeji, Adeyinka J. ^{[2
]}

Tanko, Polycarp ^{[1
]}

Gurumyen, George Y. ^{[1
]}

Buba, Deborah M. ^{[1
]}

Tizhe, Ussa D. ^{[3
]}

Bitrus, Asinamai A. ^{[1
]}

Oragwa, Arthur O. ^{[1
]}

Shaibu, Samson J. ^{[1
]}

Unanam, Essieniffiok S. ^{[4
]}

Igbokwe, Ikechukwu O. ^{[5
]}

Akpavie, Stephen O. ^{[6
]}

Njoku, Celestine O. ^{[1
]}

机构：

[1] Univ Jos, Dept Vet Microbiol & Pathol, Fac Vet Med, PMB 2084, Jos, Plateau State, Nigeria

[2] Natl Vet Res Inst, Vom, Nigeria

[3] Ahmadu Bello Univ, Dept Med, Fac Vet Med, Zaria, Nigeria

[4] Univ Jos, Dept Vet Med Surg & Radiol, Fac Vet Med, Jos, Nigeria

[5] Univ Maiduguri, Dept Vet Pathol, Fac Vet Med, Maiduguri, Nigeria

[6] Univ Ibadan, Dept Vet Pathol, Fac Vet Med, Ibadan, Nigeria

来源：

VETERINARY MEDICINE AND SCIENCE | 2021年 / 7卷 / 03期

关键词：

African swine fever; diagnosis; laboratory; Nigeria; pig; smallholder farms;

D O I：

10.1002/vms3.403

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

African swine fever (ASF) is a highly contagious fatal infectious disease of pigs and wild suids. The disease has a worldwide occurrence and significant impact on pig production. Two adult intensively raised large white boars from two farms in Jos with a history of sudden death were diagnosed of ASF between July and August 2019. Post-mortem examination of carcasses grossly showed splenomegaly, haemorrhagic lymphadenitis and hepatomegaly with severe congestion. The kidneys were enlarged and had generalized petechiae and blood clot in the pelvis. The heart was moderately enlarged. Microscopic examination of the spleen and lymph nodes revealed severe lymphocytic depletion, haemorrhage and severe haemosiderosis. The liver was severely congested with focal coagulative necrosis of the hepatocytes. The kidneys were severely congested and showed renal tubular necrosis with few tubular protein casts. Tissue samples were confirmed to be positive for African swine fever virus (ASFV) by polymerase chain reaction (PCR) assay, and phylogenetic analysis revealed that the isolate belonged to genotype I.

引用

页码：705 / 713

页数：9

共 20 条

[1] Baker J., 2000, INTRO MED LAB TECHNO, P199
[2] African swine fever: how can global spread be prevented?
Costard, Solenne
Wieland, Barbara
de Glanville, William
Jori, Ferran
Rowlands, Rebecca
Vosloo, Wilna
Roger, Francois
Pfeiffer, Dirk U.
Dixon, Linda K.
[J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 2009, 364 (1530) : 2683 - 2696
[3] African Swine Fever Epidemiology and Control
Dixon, Linda K.
Stahl, Karl
Jori, Ferran
Vial, Laurence
Pfeiffer, Dirk U.
[J]. ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 8, 2020, 2020, 8 : 221 - 246
[4] European Union Reference Laboratory for African Swine Fever, 2013, PROC AFR SWIN FEV VI, V1, P1
[5] Fadiga M., 2013, 33 ILRI NAIR
[6] FAO, 1998, FAO UN AFR SWIN FEV
[7] Surveillance for African Swine Fever in Nigeria, 2006-2009
Fasina, F. O.
Shamaki, D.
Makinde, A. A.
Lombin, L. H.
Lazarus, D. D.
Rufai, S. A.
Adamu, S. S.
Agom, D.
Pelayo, V.
Soler, A.
Simon, A.
Adedeji, A. J.
Yakubu, M. B.
Mantip, S.
Benshak, A. J.
Okeke, I.
Anagor, P.
Mandeng, D. C.
Akanbi, B. O.
Ajibade, A. A.
Faramade, I.
Kazeem, M. M.
Enurah, L. U.
Bishop, R.
Anchuelo, R.
Martin, J. H.
Gallardo, C.
[J]. TRANSBOUNDARY AND EMERGING DISEASES, 2010, 57 (04) : 244 - 253
[8] Molecular Diagnosis of African Swine Fever by a New Real-Time PCR Using Universal Probe Library
Fernandez-Pinero, J.
Gallardo, C.
Elizalde, M.
Robles, A.
Gomez, C.
Bishop, R.
Heath, L.
Couacy-Hymann, E.
Fasina, F. O.
Pelayo, V.
Soler, A.
Arias, M.
[J]. TRANSBOUNDARY AND EMERGING DISEASES, 2013, 60 (01) : 48 - 58
[9] Ganowiak J, 2012, SVERIGES LANTBRUKSUN, V57
[10] Igbokwe I. O., 2018, Revue d'Elevage et de Medecine Veterinaire des Pays Tropicaux, V71, P87

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