DNA duplex-quadruplex exchange as the basis for a nanomolecular machine

被引:331
作者
Alberti, P [1 ]
Mergny, JL [1 ]
机构
[1] CNRS, Unite Mixte Rech 8646, Lab Biophys, Museum Natl Hist Nat,Natl Natl Sante & Rech Med U, F-75005 Paris, France
关键词
molecular machine; FRET; DNA structure; G-quartet;
D O I
10.1073/pnas.0335459100
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
There is currently great interest in the design of nanodevices that are capable of performing linear or rotary movements. Protein molecular machines are abundant in biology but it has recently been proposed that nucleic acids could also act as nanomolecular machines in model systems. Several types of movements have been described with DNA machines: rotation and "scissors-like" opening and closing. Here we show a nanomachine that is capable of an extension-contraction movement. The simple and robust device described here is composed of a single 21-base oligonucleoticle and relies on a duplex-quadruplex equilibrium that may be fueled by the sequential addition of DNA single strands, generating a DNA duplex as a by-product. The interconversion between two well defined topological states induces a 5-nm two-stroke, linear motor-type movement, which is detected by fluorescence resonance energy transfer spectroscopy.
引用
收藏
页码:1569 / 1573
页数:5
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