Pseudomonas aeruginosa quorum sensing molecule N-(3 oxododecanoyl)-L-homoserine lactone disrupts epithelial barrier integrity of Caco-2 cells

被引：80

作者：

Vikstrom, Elena ^{[1
]}

Tafazoli, Farideh ^{[1
]}

Magnusson, Karl-Eric ^{[1
]}

机构：

[1] Linkoping Univ, Dept Mol & Clin Med, Div Med Microbiol, SE-58185 Linkoping, Sweden

来源：

FEBS LETTERS | 2006年 / 580卷 / 30期

关键词：

quorum sensing; epithelial barrier; tight junction; cytoskeleton; MAPK; Pseudomonas aeruginosa;

D O I：

10.1016/j.febslet.2006.11.057

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acyl-homoserine lactone (HSL) quorum sensing molecules play an important role in regulation of virulence gene expression in Pseudomonas aeruginosa. Here, we show that 30-C-12-HSL can disrupt barrier integrity in human epithelial Caco-2 cells as evidenced by decreased transepithelial electrical resistance (TER), increased paracellular flux, reduction in the expression and distribution of ZO-1 and occludin, and reorganization of F-actin. P. aeruginosa 30-C-12-HSL activate p38 and p42/44 kinases, and inhibition of these kinases partly prevented 30-C-12-HSL-induced changes in TER, paracellular flux and expression of occludin and ZO-1. These findings demonstrate that P. aeruginosa 30-C-12-HSL can modulate tight junction integrity of Caco-2 cells. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：6921 / 6928

页数：8

共 34 条

[1] A novel and sensitive method for the quantification of N-3-oxoacyl homoserine lactones using gas chromatography-mass spectrometry:: application ito a model bacterial biofilm [J].

Charlton, TS ;

de Nys, R ;

Netting, A ;

Kumar, N ;

Hentzer, M ;

Givskov, M ;

Kjelleberg, S .

ENVIRONMENTAL MICROBIOLOGY, 2000, 2 (05) :530-541

[2] Synthetic analogues of the bacterial signal (quorum sensing) molecule N-(3-oxododecanoyl)-L-homoserine lactone as immune modulators [J].

Chhabra, SR ;

Harty, C ;

Hooi, DSW ;

Daykin, M ;

Williams, P ;

Telford, G ;

Pritchard, DI ;

Bycroft, BW .

JOURNAL OF MEDICINAL CHEMISTRY, 2003, 46 (01) :97-104

[3] Inactivation of a Pseudomonas aeruginosa quorum-sensing signal by human airway epithelia [J].

Chun, CK ;

Ozer, EA ;

Welsh, MJ ;

Zabner, J ;

Greenberg, EP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (10) :3587-3590

[4] Post-transcriptional regulation of gene expression by mitogen-activated protein kinase p38 [J].

Clark, AR ;

Dean, JLE ;

Saklatvala, J .

FEBS LETTERS, 2003, 546 (01) :37-44

[5] A porous defense: the leaky epithelial barrier in intestinal disease [J].

Clayburgh, DR ;

Shen, L ;

Turner, JR .

LABORATORY INVESTIGATION, 2004, 84 (03) :282-291

[6] Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase [J].

Dong, YH ;

Wang, LH ;

Xu, JL ;

Zhang, HB ;

Zhang, XF ;

Zhang, LH .

NATURE, 2001, 411 (6839) :813-817

[7] Pseudomonas aeruginosa quorum-sensing systems may control virulence factor expression in the lungs of patients with cystic fibrosis [J].

Erickson, DL ;

Endersby, R ;

Kirkham, A ;

Stuber, K ;

Vollman, DD ;

Rabin, HR ;

Mitchell, I ;

Storey, DG .

INFECTION AND IMMUNITY, 2002, 70 (04) :1783-1790

[8] Bacterial social engagements [J].

Henke, JM ;

Bassler, BL .

TRENDS IN CELL BIOLOGY, 2004, 14 (11) :648-656

[9] Adherence to and penetration of human intestinal Caco-2 epithelial cell monolayers by Pseudomonas aeruginosa [J].

Hirakata, Y ;

Izumikawa, K ;

Yamaguchi, T ;

Igimi, S ;

Furuya, N ;

Maesaki, S ;

Tomono, K ;

Yamada, Y ;

Kohno, S ;

Yamaguchi, K ;

Kamihira, S .

INFECTION AND IMMUNITY, 1998, 66 (04) :1748-1751

[10] Quorum sensing:: the power of cooperation in the world of Pseudomonas [J].

Juhas, M ;

Eberl, L ;

Tümmler, B .

ENVIRONMENTAL MICROBIOLOGY, 2005, 7 (04) :459-471

← 1 2 3 4 →