Down-regulation of αv integrin by retroviral delivery of small interfering RNA reduces multicellular resistance of HT29

被引：21

作者：

He, Jian-ming ^{[1
]}

Wang, Feng-chao ^{[2
]}

Qi, Hua-bing ^{[2
]}

Li, Yan ^{[1
]}

Liang, Hou-jie ^{[1
]}

机构：

[1] Third Mil Med Univ, Southwest Hosp, Dept Oncol, Chongqing 400038, Peoples R China

[2] Third Mil Med Univ, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China

来源：

CANCER LETTERS | 2009年 / 284卷 / 02期

关键词：

Integrin; Oxaliplatin; Chemotherapy; Multicellular resistance; Colon cancer; NF-KAPPA-B; GROWTH-FACTOR; CELL-SURVIVAL; SHEAR-STRESS; E-CADHERIN; ACTIVATION; INTEGRIN-ALPHA(V)BETA(3); EXPRESSION; ADHESION; MATRIX;

D O I：

10.1016/j.canlet.2009.04.023

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Since multicellular resistance (MCR) has been shown to be as adhesion-dependent, the role of alpha v integrin in MCR of HT29 was investigated in this paper. Down-regulation of alpha v integrin reduced MCR to oxaliplatin, but did not detectably change the drug sensitivity of monolayers. Down-regulation of alpha v integrin decreased phosphorylated NF-kappa B p65 and increased phosphorylated JNK2 in multicellular spheroids. Cell-cell adhesion and cell-cell junctions in multicellular spheroids resembled the in vivo situation. Since force, including adhesion, can activate alpha v integrin, cell-cell contact may contribute to activation of alpha v integrin, through which increasing phosphorylated p65 and decreasing phosphorylated JNK2 takes part in MCR. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：182 / 188

页数：7

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