Mdmx enhances p53 ubiquitination by altering the substrate preference of the Mdm2 ubiquitin ligase

被引：70

作者：

Okamoto, Koji ^{[1
,2
,3
]}

Taya, Yoichi ^{[2
,3
]}

Nakagama, Hitoshi ^{[1
]}

机构：

[1] Natl Canc Ctr, Res Inst, Early Oncogenesis Res Project, Chuo Ku, Tokyo 1040045, Japan

[2] Natl Canc Ctr, Res Inst, Div Radiobiol, Chuo Ku, Tokyo 1040045, Japan

[3] Japan Sci & Technol Corp, SORST, Tokyo, Japan

来源：

FEBS LETTERS | 2009年 / 583卷 / 17期

关键词：

Mdmx; Mdm2; p53; Ubiquitination; NUCLEAR EXPORT; ONCOPROTEIN MDM2; PROTEIN; DOMAIN; ACETYLATION; DEGRADATION; ACTIVATION; NETWORK; PATHWAY; COMPLEX;

D O I：

10.1016/j.febslet.2009.07.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

mdm2 and mdmx oncogenes play essential yet non-redundant roles in synergistic inactivation of the tumor suppressor, p53. While Mdm2 inhibits p53 activity mainly by augmenting its ubiquitination, the functional role of Mdmx on p53 ubiquitination remains obscure. In transfected H1299 cells, Mdmx augmented Mdm2-mediated ubiquitination of p53. In in vitro ubiquitination assays, the Mdmx/Mdm2 heteromeric complex, in comparison to the Mdm2 homomer, showed enhanced ubiquitinase activity toward p53 and the reduced auto-ubiquitination of Mdm2. Alteration of the substrate specificity via binding to Mdmx may contribute to efficient ubiquitination and inactivation of p53 by Mdm2. Structured summary: MINT-7219995: P53 (uniprotkb: P04637) physically interacts (MI: 0914) with Ubiquitin ( uniprotkb: P62988) by anti bait coimmunoprecipitation ( MI: 0006) MINT-7220023: Ubiquitin ( uniprotkb: P62988) physically interacts ( MI: 0914) with P53 ( uniprotkb: P04637) by pull down ( MI: 0096) (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：2710 / 2714

页数：5

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