Characterization of the complete chloroplast genome of the Chinese yew Taxus chinensis (Taxaceae), an endangered and medicinally important tree species in China

被引：9
作者：
Jia, Xiao-Ming [1 ]
Liu, Xi-Ping [2 ]
机构：
[1] Northwest A&F Univ, Dept Forestry, Coll Forestry, Yangling 712100, Shaanxi Provinc, Peoples R China
[2] Northwest A&F Univ, Dept Plant Physiol, Coll Life Sci, 3 Taicheng Rd, Yangling 712100, Shaanxi Provinc, Peoples R China
来源：
CONSERVATION GENETICS RESOURCES | 2017年 / 9卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Taxus chinensis; Chloroplast genome; High-throughput sequencing; MITObim; MITOCHONDRIAL GENOMES; PACLITAXEL; CULTURES;
D O I：
10.1007/s12686-016-0649-1
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
The Chinese yew Taxus chinensis (Taxaceae) is an endangered tree species native to China, and possesses high medicinal values. To facilitate its conservation genetics studies, its complete chloroplast genome was characterized using Illumina high-throughput sequencing technology in this study. The circular genome is 127,743 bp in length with an asymmetric base composition (32.6% A, 17.7% C, 17.0% G and 32.7% T). It harbors a total of 113 genes, including 82 protein-coding genes (PCGs), 27 tRNAs and four rRNAs. Gene duplication was detected for a couple of genes (trnG-UCC and trnQ-UUG). Besides, seven genes (atpF, ndhA, ndhB, ropC1, rpl2, trnK-UUU and trnL-UAA) harbor a single intron, and another gene (ycf3) possesses a couple of introns. Phylogenetic analysis suggests that T. chinensis and T. mairei are closely related to each other.
引用
收藏
页码：197 / 199
页数：3
相关论文
共 11 条
[1]  
Futuyma D. J., 1986, EVOL BIOL
[2]   Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads-a baiting and iterative mapping approach [J].
Hahn, Christoph ;
Bachmann, Lutz ;
Chevreux, Bastien .
NUCLEIC ACIDS RESEARCH, 2013, 41 (13) :e129
[3]   OrganellarGenomeDRAW-a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets [J].
Lohse, Marc ;
Drechsel, Oliver ;
Kahlau, Sabine ;
Bock, Ralph .
NUCLEIC ACIDS RESEARCH, 2013, 41 (W1) :W575-W581
[4]   A large-scale purification of paclitaxel from cell cultures of Taxus chinensis [J].
Pyo, SH ;
Park, HB ;
Song, BK ;
Han, BH ;
Kim, JH .
PROCESS BIOCHEMISTRY, 2004, 39 (12) :1985-1991
[5]  
State Forestry Administration of P. R. China Ministry of Agriculture of P. R. China, 1999, LIST STATE PROTECTED
[6]  
Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI [10.1093/molbev/mst197, 10.1093/molbev/msr121]
[7]  
Thomas P, 2013, IUCN RED LIST THREAT, V2013, DOI [10.2305/IUCN.UK.2013-1.RLTS.T42548A2987120.en, DOI 10.2305/IUCN.UK.2013-1.RLTS.T42548A2987120.EN]
[8]   Enhanced paclitaxel production induced by the combination of elicitors in cell suspension cultures of Taxus chinensis [J].
Zhang, CH ;
Mei, XG ;
Liu, L ;
Yu, LJ .
BIOTECHNOLOGY LETTERS, 2000, 22 (19) :1561-1564
[9]  
Zhang XueMei Zhang XueMei, 2012, Journal of Southwest Forestry University, V32, P92
[10]   The complete chloroplast genome sequence of Taxus chinensis var. mairei (Taxaceae): loss of an inverted repeat region and comparative analysis with related species [J].
Zhang, Yanzhen ;
Ma, Ji ;
Yang, Bingxian ;
Li, Ruyi ;
Zhu, Wei ;
Sun, Lianli ;
Tian, Jingkui ;
Zhang, Lin .
GENE, 2014, 540 (02) :201-209
12