Fibro-C-Index: comprehensive, morphology-based quantification of liver fibrosis using second harmonic generation and two-photon microscopy

被引：79

作者：

Tai, Dean C. S. ^{[1
]}

Tan, Nancy ^{[2
,3
]}

Xu, Shuoyu ^{[1
,5
]}

Kang, Chiang Huen ^{[1
]}

Chia, Ser Mien ^{[5
]}

Cheng, Chee Leong ^{[6
]}

Wee, Aileen ^{[6
]}

Wei, Chiang Li ^{[4
]}

Raja, Anju Mythreyi ^{[1
,7
]}

Xiao, Guangfa ^{[2
,8
]}

Chang, Shi ^{[8
]}

Rajapakse, Jagath C. ^{[5
,9
,10
]}

机构：

[1] Inst Bioengn & Nanotechnol, Singapore 138669, Singapore

[2] Natl Univ Singapore, Dept Physiol, Singapore 117597, Singapore

[3] KK Womens & Childerns Hosp, Dept Paediat, Singapore 229899, Singapore

[4] KK Womens & Childerns Hosp, Dept Paediat Surg, Singapore 229899, Singapore

[5] Natl Univ Singapore, Computat & Syst Biol Program, Singapore MIT Alliance, Singapore 117576, Singapore

[6] Natl Univ Singapore Hosp, Dept Pathol, Singapore 119074, Singapore

[7] Natl Univ Singapore, Grad Sch Integrat Sci & Engn, Grad Programme Bioengn, Singapore 117597, Singapore

[8] Cent S Univ, Xiangya Hosp, Dept Gen Surg, Changsha 410008, Hunan, Peoples R China

[9] Nanyang Technol Univ, Sch Comp Engn, Bioinformat Res Ctr, Singapore 639798, Singapore

[10] MIT, Div Biol Engn, Cambridge, MA 02139 USA

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2009年 / 14卷 / 04期

基金：

英国医学研究理事会;

关键词：

liver fibrosis; second harmonic generation; bioimaging; diagnosis; two photon; medical optics; CHRONIC HEPATITIS-C; IMAGE-ANALYSIS METHOD; VIRUS-INFECTION; OBSERVER VARIATION; COLLAGEN; INTEROBSERVER; BIOPSIES; FLUORESCENCE; ORIENTATION; CIRRHOSIS;

D O I：

10.1117/1.3183811

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We develop a standardized, fully automated, quantification system for liver fibrosis assessment using second harmonic generation microscopy and a morphology-based quantification algorithm. Liver fibrosis is associated with an abnormal increase in collagen as a result of chronic liver diseases. Histopathological scoring is the most commonly used method for liver fibrosis assessment, where a liver biopsy is stained and scored by experienced pathologists. Due to the intrinsic limited sensitivity and operator-dependent variations, there exist high inter-and intraobserver discrepancies. We validate our quantification system, Fibro-C-Index, with a comprehensive animal study and demonstrate its potential application in clinical diagnosis to reduce inter-and intraobserver discrepancies. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3183811]

引用

页数：10

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