OMSSA Parser: An open-source library to parse and extract data from OMSSA MS/MS search results

被引:26
作者
Barsnes, Harald [1 ]
Huber, Steffen [2 ,3 ]
Sickmann, Albert [2 ]
Eidhammer, Ingvar [1 ]
Martens, Lennart [4 ]
机构
[1] Univ Bergen, Dept Informat, N-5020 Bergen, Norway
[2] ISAS Inst Analyt Sci, Dortmund, Germany
[3] Tech Univ Dresden, Dresden, Germany
[4] EBI, EMBL Outstn, Cambridge, England
关键词
Bioinformatics; OMSSA; Protein identification; PEPTIDE IDENTIFICATIONS; MASS;
D O I
10.1002/pmic.200900037
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Protein identification by MS is an important technique in both gel-based and gel-free proteome studies. The Open Mass Spectrometry Search Algorithm (OMSSA) (http:// pubchem.ncbi.nlm.nih.gov/omssa) is an open-source search engine that can be used to identify MS/MS spectra acquired in these experiments. We here present a lightweight, open-source Java software library, OMSSA Parser (http://code.google.com/p/omssa-parser), which parses OMSSA omx result files into easy accessible and fully functional object models. In addition, we also provide examples illustrating the usage of our library.
引用
收藏
页码:3772 / 3774
页数:3
相关论文
共 14 条
  • [1] Mass spectrometry-based proteomics
    Aebersold, R
    Mann, M
    [J]. NATURE, 2003, 422 (6928) : 198 - 207
  • [2] AN APPROACH TO CORRELATE TANDEM MASS-SPECTRAL DATA OF PEPTIDES WITH AMINO-ACID-SEQUENCES IN A PROTEIN DATABASE
    ENG, JK
    MCCORMACK, AL
    YATES, JR
    [J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (11) : 976 - 989
  • [3] A method for assessing the statistical significance of mass spectrometry-based protein identifications using general scoring schemes
    Fenyö, D
    Beavis, RC
    [J]. ANALYTICAL CHEMISTRY, 2003, 75 (04) : 768 - 774
  • [4] Open mass spectrometry search algorithm
    Geer, LY
    Markey, SP
    Kowalak, JA
    Wagner, L
    Xu, M
    Maynard, DM
    Yang, XY
    Shi, WY
    Bryant, SH
    [J]. JOURNAL OF PROTEOME RESEARCH, 2004, 3 (05) : 958 - 964
  • [5] MascotDatfile: An open-source library to fully parse and analyse MASCOT MS/MS search results
    Helsens, Kenny
    Martens, Lennart
    Vandekerckhove, Joel
    Gevaert, Kris
    [J]. PROTEOMICS, 2007, 7 (03) : 364 - 366
  • [6] Peptizer, a Tool for Assessing False Positive Peptide Identifications and Manually Validating Selected Results
    Helsens, Kenny
    Timmerman, Evy
    Vandekerckhove, Joel
    Gevaert, Kris
    Martens, Lennart
    [J]. MOLECULAR & CELLULAR PROTEOMICS, 2008, 7 (12) : 2364 - 2372
  • [7] Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search
    Keller, A
    Nesvizhskii, AI
    Kolker, E
    Aebersold, R
    [J]. ANALYTICAL CHEMISTRY, 2002, 74 (20) : 5383 - 5392
  • [8] RScore: a peptide randomicity score for evaluating tandem mass spectra
    Li, FX
    Sun, W
    Gao, YH
    Wang, J
    [J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (14) : 1655 - 1659
  • [9] PRIDE: The proteomics identifications database
    Martens, L
    Hermjakob, H
    Jones, P
    Adamski, M
    Taylor, C
    States, D
    Gevaert, K
    Vandekerckhove, J
    Apweiler, R
    [J]. PROTEOMICS, 2005, 5 (13) : 3537 - 3545
  • [10] MATTHIESEN R, 2007, METHOD MOL BIOL, P121