Cell-derived extracellular vesicles can be used as a biomarker reservoir for glioblastoma tumor subtyping

被引：81

作者：

Lane, Rosemary ^{[1
]}

Simon, Thomas ^{[1
]}

Vintu, Marian ^{[1
]}

Solkin, Benjamin ^{[1
]}

Koch, Barbara ^{[1
]}

Stewart, Nicolas ^{[2
]}

Benstead-Hume, Graeme ^{[3
]}

Pearl, Frances M. G. ^{[3
]}

Critchley, Giles ^{[4
]}

Stebbing, Justin ^{[5
]}

Giamas, Georgios ^{[1
]}

机构：

[1] Univ Sussex, Sch Life Sci, Dept Biochem & Biomed, Brighton BN1 9QG, E Sussex, England

[2] Univ Brighton, Pharm & Biomol Sci, Brighton BN2 4GJ, E Sussex, England

[3] Univ Sussex, Sch Life Sci, Bioinformat Grp, Brighton BN1 9QG, E Sussex, England

[4] Brighton & Sussex Univ Hosp, Dept Neurosurg, Hurstwood Pk Neurosci Ctr, Brighton, E Sussex, England

[5] Imperial Coll London, Dept Surg & Canc, Div Canc, Hammersmith Hosp Campus, Du Cane Rd, London W12 ONN, England

来源：

COMMUNICATIONS BIOLOGY | 2019年 / 2卷 / 1期

基金：

英国惠康基金;

关键词：

PROTEOME; LINES; IDENTIFICATION; BEVACIZUMAB; PROGRESSION; EXPRESSION; LANDSCAPE; PHENOTYPE; SIGNATURE; EVOLUTION;

D O I：

10.1038/s42003-019-0560-x

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Glioblastoma (GBM) is one of the most aggressive solid tumors for which treatment options and biomarkers are limited. Small extracellular vesicles (sEVs) produced by both GBM and stromal cells are central in the inter-cellular communication that is taking place in the tumor bulk. As tumor sEVs are accessible in biofluids, recent reports have suggested that sEVs contain valuable biomarkers for GBM patient diagnosis and follow-up. The aim of the current study was to describe the protein content of sEVs produced by different GBM cell lines and patient-derived stem cells. Our results reveal that the content of the sEVs mirrors the phenotypic signature of the respective GBM cells, leading to the description of potential informative sEV-associated biomarkers for GBM subtyping, such as CD44. Overall, these data could assist future GBM in vitro studies and provide insights for the development of new diagnostic and therapeutic methods as well as personalized treatment strategies.

引用

页数：12

共 65 条

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