Effects of sulfated lentinan on cellular infectivity of avian infectious bronchitis virus

被引：24

作者：

Wang, Deyun ^{[1
]}

Guo, Zhenhuan ^{[1
]}

Ma, Xia ^{[1
]}

Hu, Yuanliang ^{[1
]}

Huang, Xiaoyan ^{[1
]}

Fan, Yunpeng ^{[1
]}

Yang, Shujuan ^{[1
]}

Guo, Liwei ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Inst Tradit Chinese Vet Med, Nanjing 210095, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2010年 / 79卷 / 02期

关键词：

Lentinan; Sulfated modification; Infectious bronchitis virus; Chicken embryo fibroblast (CEF); Cellular infectivity; POLYSACCHARIDE;

D O I：

10.1016/j.carbpol.2009.09.016

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In order to investigate the possibility of sulfating modification in improving the anti-viral activity of lentinan (LNT), three kinds of sulfated LNTs (sLNT), sLNT(1), sLNT(2) and sLNT(3) with the degree of substitution of 0.69, 0.98 and 1.37 were prepared by chlorosulfonic acid-pyridine method with three modification condition, respectively. The sLNTs and avain infectious bronchitis virus (IBV) were added into chicken embryo fibroblast (CEF) in three manners, pre-adding polysaccharide, post-adding polysaccharide and mixed adding polysaccharide with IBV, respectively, taking non-modified LNT as control. The anti-viral activities of three sLNTs were compared by MTT method. The results showed that all of sLNTs and LNT at a certain concentration could significantly inhibit IBV growth in three manners. The effects of modified sLNTs were better than that of non-modified LNT. It indicated that sulfated modification could enhance the anti-viral activity of LNT. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：461 / 465

页数：5

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