The Effect of Surface Treatment of Silicone Hydrogel Contact Lenses on the Attachment of Acanthamoeba castellanii Trophozoites

被引:21
作者
Beattie, Tara K. [1 ]
Tomlinson, Alan [2 ]
机构
[1] Univ Strathclyde, Dept Civil Engn, Glasgow G4 ONG, Lanark, Scotland
[2] Glasgow Caledonian Univ, Dept Vis Sci, Glasgow G4 0BA, Lanark, Scotland
来源
EYE & CONTACT LENS-SCIENCE AND CLINICAL PRACTICE | 2009年 / 35卷 / 06期
关键词
Acanthamoeba; Contact lenses; Keratitis; Eye infection; MICROBIAL KERATITIS; RISK; CONTAMINATION; EPIDEMIOLOGY; WEAR;
D O I
10.1097/ICL.0b013e3181becce6
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Objective: To determine if plasma surface treatment of Focus Night & Day silicone hydrogel contact lenses affects the attachment of Acanthamoeba. Methods: Unworn lotrafilcon A contact lenses with (Focus Night & Day) and without surface treatment and Acuvue, conventional hydrogel lenses, were quartered before 90-min incubation with Acanthamoeba castellanii trophozoites. After incubation and rinsing, the trophozoites attached to one surface of each quarter were counted by direct light microscopy. Sixteen replicates were observed for each lens type. Logarithmic transformation of data allowed the use of parametric analysis of variance. Results: No significant difference in attachment was established between the untreated lotrafilcon A and the conventional hydrogel lenses (P<0.001); however, surface treatment of the native Focus Night & Day material produced a significant increase in attachment (P<0.001). Conclusions: Commercially available Focus Night & Day lenses are subjected to a plasma surface treatment to reduce lens hydrophobicity; however, this procedure results in an enhanced acanthamoebal attachment. It is possible that the silicone hydrogel lens could be at a greater risk of promoting Acanthamoeba infection if exposed to the organism because of the enhanced attachment characteristic of this material. Eye care professionals should be aware of the enhanced affinity that Acanthamoeba show for this lens and accordingly emphasise to patients the significance of appropriate lens hygiene. This is particularly important where lenses are worn in a regime that could increase the chance of exposure to the organism, i.e., 6 nights/7 days extended wear or daily wear, where lenses will be stored in a lens case, or where lenses are worn when in contact with potentially contaminated water sources, i.e., swimming or showering.
引用
收藏
页码:316 / 319
页数:4
相关论文
共 38 条
[1]  
Beattie Tara K, 2003, Eye Contact Lens, V29, pS40
[2]   Attachment of Acanthamoeba to first- and second-generation silicone hydrogel contact lenses [J].
Beattie, TK ;
Tomlinson, A ;
McFadyen, AK .
OPHTHALMOLOGY, 2006, 113 (01) :117-125
[3]   Enhanced attachment of Acanthamoeba to extended-wear silicone hydrogel contact lenses -: A new risk factor for infection.? [J].
Beattie, TK ;
Tomlinson, A ;
McFadyen, AK ;
Seal, DV ;
Grimason, AM .
OPHTHALMOLOGY, 2003, 110 (04) :765-771
[4]  
BEATTIE TK, 2006, INVEST OPHTHALMOL VI, V47
[5]  
Borazjani Roya N, 2005, Eye Contact Lens, V31, P39, DOI 10.1097/01.ICL.0000146303.00326.69
[6]   Twenty Years of Acanthamoeba Keratitis [J].
Carvalho, F. R. S. ;
Foronda, A. S. ;
Mannis, M. J. ;
Hoefling-Lima, A. L. ;
Belfort, R., Jr. ;
de Freitas, Denise .
CORNEA, 2009, 28 (05) :516-519
[7]  
Centers for Disease Control and Prevention (CDC), 2007, MMWR Morb Mortal Wkly Rep, V56, P532
[8]   Risk factors for microbial keratitis with contemporary contact lenses - A case-control study [J].
Dart, J. K. G. ;
Radford, C. F. ;
Minassian, D. ;
Verma, S. ;
Stapleton, F. .
OPHTHALMOLOGY, 2008, 115 (10) :1647-1654
[9]   MICROBIAL-CONTAMINATION OF CONTACT-LENS CASES IN THE WEST OF SCOTLAND [J].
DEVONSHIRE, P ;
MUNRO, FA ;
ABERNETHY, C ;
CLARK, BJ .
BRITISH JOURNAL OF OPHTHALMOLOGY, 1993, 77 (01) :41-45
[10]  
GUPTA S, 2009, INVEST OPHTHALMOL VI, V50