Tetrahydrobiopterin as a mediator of PC12 cell proliferation induced by EGF and NGF

被引：34

作者：

Anastasiadis, PZ

Bezin, L

Imerman, BA

Kuhn, DM

Louie, MC

Levine, RA

机构：

[1] WAYNE STATE UNIV,SCH MED,DEPT PSYCHIAT & BEHAV NEUROSCI,DETROIT,MI 48201

[2] VET ADM MED CTR,DETROIT,MI 48201

[3] HENRY FORD HOSP,WILLIAM T GOSSETT NEUROL LABS,DETROIT,MI 48202

来源：

EUROPEAN JOURNAL OF NEUROSCIENCE | 1997年 / 9卷 / 09期

关键词：

actinomycin D; cAMP; GTP cyclohydrolase; rat;

D O I：

10.1111/j.1460-9568.1997.tb00749.x

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Epidermal growth factor and nerve growth factor increased the proliferation of rat phaeochromocytoma PC12 cells through obligatory elevation of intracellular (6R)-tetrahydrobiopterin (BH4). Epidermal growth factor and nerve growth factor increased intracellular BH4 by inducing GTP-cyclohydrolase, the rate-limiting enzyme in BH4 biosynthesis. Specific inhibitors of BH4 biosynthesis prevented growth factor-induced increases in BH4 levels and proliferation. The induction of GTP cyclohydrolase, BH4 and cellular proliferation by nerve growth factor was mediated by cAMP. Elevation of BH4 biosynthesis occurred downstream from cAMP in the cascade used by nerve growth factor to increase proliferation. Thus, intracellular BH4 is an essential mediator of the proliferative effects of epidermal growth factor and nerve growth factor in PC12 cells.

引用

页码：1831 / 1837

页数：7

共 41 条

[1]

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[2]

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