The Effect of Ozone Treatment on Thermal Burn Wound Healing; an Experimental Study

被引:2
作者
Peker, Kemal [1 ]
Yilmaz, Ismayil [2 ]
Demiryilmaz, Ismail [3 ]
Isik, Arda [4 ]
Sayar, Ilyas [5 ]
Gursul, Cebrail [6 ]
Cankaya, Murat [7 ]
Coban, Taha Abdulkadir [8 ]
机构
[1] Duzce Univ, Dept Gen Surg, Duzce, Turkey
[2] Minist Hlth Univ, Gen Surg Dept, Ankara, Turkey
[3] Akdeniz Univ, Dept Gen Surg, Antalya, Turkey
[4] Erzincan Univ, Dept Gen Surg, Erzincan, Turkey
[5] Erzincan Univ, Dept Pathol, Erzincan, Turkey
[6] Erzincan Univ, Dept Physiol, Erzincan, Turkey
[7] Erzincan Univ, Dept Biol, Erzincan, Turkey
[8] Erzincan Univ, Dept Biochem, Erzincan, Turkey
来源
KONURALP TIP DERGISI | 2020年 / 12卷 / 03期
关键词
Wound Healing; Hydroxproline; Burn; LIPID-PEROXIDATION; 6-PHOSPHOGLUCONATE DEHYDROGENASE; THERAPY; INJURY; SKIN; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE; EFFICACY;
D O I
10.18521/ktd.706218
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: Ozone has been advised as a metabolic excitative in cell, immunomodulatory agent and antioxidant enzyme actuator. The present study was performed to designate the profit of ozone therapy on the burn wound healing in the rats. Methods: 40 adult Wistar type rats divided into 4 groups: a control group (burn not created and topical and systemic agents not used), a burn group (burn created but only saline given), a burn+silver sulfadiazine group (silver sulfadiazine was applied for 21 days), a burn+ozone/oxygen mixture group (ozone/oxygen mixture was applied for 21 days).After anesthetizing, second-degree burn (2 cm2 areas) was done on the dorsal of the animals byaluminum plate in boiling hot water (100 degrees C for 15 seconds) and other groups except control group were treated topically, based on the time scheduled. Tissue samples were harvested on day 7st and 21st after burn injury. Biochemical and histological analyzes were performed in tissue and blood samples. The results were assessed with appropriate statistical tests and given as Means +/- SD. Results: The histopathologic damage level was significantly different in all groups. Fibrosis and inflamation levels decreased in ozone treatment and silver sulphadiazine groups compared to burn+normal saline group on the 21 days according to 7 days. In the ozone treatment group, Glucose 6-phosphate dehydrogenase activities were significantly higher than the silver sulfadiazine treated group. But glutathione reductase enzyme activities were lover in the ozone treated group and hydroxyproline concentration decreased in ozone group compared to burn+normal saline group on 7 and 21 days. Conclusions: Ozone has a detractive effect in the development of inflamation, fibrosis, and granulation via decreasing tissue damage and increasing the antioxidant enzyme activity on burn wound healing.
引用
收藏
页码:511 / 518
页数:8
相关论文
共 43 条
[1]  
[Anonymous], 2010, DERMATOZ
[2]   Pathophysiology of the burn wound and pharmacological treatment. The Rudi Hermans lecture, 1995 [J].
Arturson, G .
BURNS, 1996, 22 (04) :255-274
[3]   Human glucose-6-phosphate dehydrogenase:: the crystal structure reveals a structural NADP+ molecule and provides insights into enzyme deficiency [J].
Au, SWN ;
Gover, S ;
Lam, VMS ;
Adams, MJ .
STRUCTURE, 2000, 8 (03) :293-303
[4]  
Beutler E., 1971, RED CELL METABOLISM
[6]  
BOCCI V, 1990, HAEMATOLOGICA, V75, P510
[7]  
Bocci V, 1996, J BIOL REG HOMEOS AG, V10, P31
[8]  
Bocci V, 1999, BRIT J BIOMED SCI, V56, P270
[9]  
Bocci VA, 2006, ARCH MED RES, V37, P425, DOI 10.1016/j.arcmed.2005.08.006
[10]   ACCELERATION OF TENSILE-STRENGTH OF INCISIONS TREATED WITH EGF AND TGF-BETA [J].
BROWN, GL ;
CURTSINGER, LJ ;
WHITE, M ;
MITCHELL, RO ;
PIETSCH, J ;
NORDQUIST, R ;
VONFRAUNHOFER, A ;
SCHULTZ, GS .
ANNALS OF SURGERY, 1988, 208 (06) :788-794