Expression and stability of two isoforms of ABCG1 in human vascular cells

被引：28

作者：

Gelissen, Ingrid C. ^{[2
]}

Cartland, Sian ^{[2
]}

Brown, Andrew J. ^{[3
]}

Sandoval, Cecilia ^{[2
]}

Kim, Mijurng ^{[2
]}

Dinnes, Donna Lee ^{[2
]}

Lee, Youra ^{[2
]}

Hsieh, Victar ^{[2
]}

Gaus, Katharina ^{[2
]}

Kritharides, Leonard ^{[2
,4
]}

Jessup, Wendy ^{[1
,2
]}

机构：

[1] Univ New S Wales, Sch Med Sci, Ctr Vasc Res, Sydney, NSW 2052, Australia

[2] Prince Wales Hosp, Dept Haematol, Sydney, NSW, Australia

[3] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW, Australia

[4] Concord Repatriat Gen Hosp, Dept Cardiol, Sydney, NSW, Australia

来源：

ATHEROSCLEROSIS | 2010年 / 208卷 / 01期

基金：

英国医学研究理事会;

关键词：

ABC transporter; Reverse cholesterol transport; Macrophage; Vascular endothelial cells; Smooth muscle cells; CASSETTE TRANSPORTER G1; HIGH-DENSITY-LIPOPROTEIN; DROSOPHILA-WHITE GENE; ATHEROSCLEROTIC LESION DEVELOPMENT; CELLULAR CHOLESTEROL EFFLUX; FATTY-ACIDS PHOSPHORYLATE; TYPE-2; DIABETES-MELLITUS; ENDOTHELIAL-CELLS; LIPID-ACCUMULATION; DESTABILIZE ABCA1;

D O I：

10.1016/j.atherosclerosis.2009.06.028

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective: To evaluate the expression of two ABCG1 isoforms that differ in the presence or absence of a 12 amino acid (AA) peptide between the ABC cassette and the transmembrane region, termed ABCG1(+ 12) and ABCG1(-12), respectively, in human vascular cells and tissues. Methods and results: mRNA for both isoforms was expressed in human macrophages, vascular endothelial and smooth muscle cells as well as whole human spleen, lung, liver and brain tissue. However, ABCG1(+ 12) was not expressed in mouse tissues. 2D gel electrophoresis of ABCG1 protein indicated that both protein isoforms were expressed in human macrophages. Furthermore the half-lives of the two ABCG1 protein isoforms, stably expressed in CHOK1 cells, measured under basal conditions were different, suggesting the presence of a degradation or stabilising signal in or near the 12AA region of ABCG1(+ 12). Conclusion: ABCG1(+ 12) is an isoform of ABCG1 exclusively expressed in human cells at the RNA and protein level. As ABCG1(+ 12) is not expressed in mice, although mouse models are widely used to elucidate the function of ABCG1, further investigations into the importance of this human ABCG1 isoform are warranted. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

引用

页码：75 / 82

页数：8

共 35 条

[1] Deletion of the transmembrane transporter ABCG1 results in progressive pulmonary lipidosis [J].

Baldan, Angel ;

Tarr, Paul ;

Vales, Charisse S. ;

Frank, Joy ;

Shimotake, Thomas K. ;

Hawgood, Sam ;

Edwards, Peter A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (39) :29401-29410

[2]

Chen HM, 1996, AM J HUM GENET, V59, P66

[3] Isolation and characterization of a mammalian homolog of the Drosophila white gene [J].

Croop, JM ;

Tiller, GE ;

Fletcher, JA ;

Lux, ML ;

Raab, E ;

Goldenson, D ;

Son, D ;

Arciniegas, S ;

Wu, RL .

GENE, 1997, 185 (01) :77-85

[4] Is two out of three enough for ABCG1? [J].

Curtiss, Linda K. .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2006, 26 (10) :2175-2177

[5] Feedback regulation of cholesterol synthesis: sterol-accelerated ubiquitination and degradation of HMG CoA reductase [J].

DeBose-Boyd, Russell A. .

CELL RESEARCH, 2008, 18 (06) :609-619

[6] LXR-induced reverse cholesterol transport in human airway smooth muscle is mediated exclusively by ABCA1 [J].

Delvecchio, Christopher J. ;

Bilan, Patricia ;

Nair, Parameswaran ;

Capone, John P. .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2008, 295 (05) :L949-L957

[7] Expression and functional characterization of ABCG1 splice variant ABCG1(666) [J].

Engel, Thomas ;

Bode, Guenther ;

Lueken, Aloys ;

Knop, Markus ;

Kannenberg, Frank ;

Nofer, Jerzy-Roch ;

Assmann, Gerd ;

Seedorf, Udo .

FEBS LETTERS, 2006, 580 (18) :4551-4559

[8] A kinetic model to evaluate cholesterol efflux from THP-1 macrophages to apolipoprotein a-1 [J].

Gaus, K ;

Gooding, JJ ;

Dean, RT ;

Kritharides, L ;

Jessup, W .

BIOCHEMISTRY, 2001, 40 (31) :9363-9373

[9]

Gelissen IC, 1999, J LIPID RES, V40, P1636

[10] ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I [J].

Gelissen, IC ;

Harris, M ;

Rye, KA ;

Quinn, C ;

Brown, AJ ;

Kockx, M ;

Cartland, S ;

Packianathan, M ;

Kritharides, L ;

Jessup, W .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2006, 26 (03) :534-540

← 1 2 3 4 →