Synthesis and Evaluation of Novel Isoindoline-1,3-dione Derivatives as Anticancer Agents

N-Substituted imides, isoindoline-1,3-dione derivatives, were synthesized, characterized, and investigated against blood cancer using K562 and Raji cell lines. Cytotoxicity assay was performed to determine the influence of phthalimide derivatives on the survival of the cancer cells. In addition, flow-cytometry with annexin-V-conjugated with fluorescein isothiocyanate (FITC) and propidium iodide (PI) stains were used to investigate the type of cell death induced by our phthalimide derivatives. 2-(4-(2-Bromoacetyl)phenyl)isoindoline-1,3-dione showed the most inhibitory effect on the viability of the cancer cells (CC50 = 0.26 mu g/mL for Raji cells and 3.81 mu g/mL for K562 cells). As a result, 2-(4-(2-bromoacetyl)phenyl)isoindoline-1,3-dione was selected for further investigations to determine the type of cellular death against Raji cells. The analysis found that this compound induced apoptosis and necrosis in Raji cells. Our findings provide a basis for a further study to investigate the impact of additional alkylating imides on the survival of blood cancer cells, particularly against Raji cells.