The Combination of Bromelain and Acetylcysteine (BromAc) Synergistically Inactivates SARS-CoV-2

被引：29

作者：

Akhter, Javed ^{[1
,2
]}

Queromes, Gregory ^{[3
]}

Pillai, Krishna ^{[2
]}

Kepenekian, Vahan ^{[1
,4
]}

Badar, Samina ^{[1
,5
]}

Mekkawy, Ahmed H. ^{[1
,2
,5
]}

Frobert, Emilie ^{[3
,6
]}

Valle, Sarah J. ^{[1
,2
,5
]}

Morris, David L. ^{[1
,2
,5
]}

机构：

[1] St George Hosp, Dept Surg, Sydney, NSW 2217, Australia

[2] Mucpharm Pty Ltd, Sydney, NSW 2217, Australia

[3] Univ Claude Bernard Lyon 1, Univ Lyon, INSERM,U1111,ENS Lyon, CIRI,Ctr Int Rech Infectiol,Team VirPatH,CNRS,UMR, F-69007 Lyon, France

[4] Lyon 1 Univ, Hosp Civils Lyon, EMR 3738, CICLY, F-69921 Lyon, France

[5] Univ New South Wales, St George & Sutherland Clin Sch, Sydney, NSW 2217, Australia

[6] Grp Hosp Nord, Hosp Civils Lyon, Inst Agents Infect IAI, Lab Virol, F-69004 Lyon, France

来源：

VIRUSES-BASEL | 2021年 / 13卷 / 03期

关键词：

SARS-CoV-2; Bromelain; Acetylcysteine; BromAc; drug repurposing;

D O I：

10.3390/v13030425

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Severe acute respiratory syndrome coronavirus (SARS-CoV-2) infection is the cause of a worldwide pandemic, currently with limited therapeutic options. The spike glycoprotein and envelope protein of SARS-CoV-2, containing disulfide bridges for stabilization, represent an attractive target as they are essential for binding to the ACE2 receptor in host cells present in the nasal mucosa. Bromelain and Acetylcysteine (BromAc) has synergistic action against glycoproteins by breakage of glycosidic linkages and disulfide bonds. We sought to determine the effect of BromAc on the spike and envelope proteins and its potential to reduce infectivity in host cells. Recombinant spike and envelope SARS-CoV-2 proteins were disrupted by BromAc. Spike and envelope protein disulfide bonds were reduced by Acetylcysteine. In in vitro whole virus culture of both wild-type and spike mutants, SARS-CoV-2 demonstrated a concentration-dependent inactivation from BromAc treatment but not from single agents. Clinical testing through nasal administration in patients with early SARS-CoV-2 infection is imminent.

引用

页数：11

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