Pulmonary artery smooth muscle cell [Ca2+](i) and contraction: responses to diphenyleneiodonium and hypoxia

被引:40
作者
Zhang, F
Carson, RC
Zhang, H
Gibson, G
Thomas, HM
机构
[1] BURKE MED RES INST, WILL ROGERS PULM RES LAB, WHITE PLAINS, NY 10605 USA
[2] CORNELL UNIV, COLL MED, WILL ROGERS PULM RES LAB, DIV PULM & CRIT CARE MED, WHITE PLAINS, NY 10605 USA
[3] CORNELL UNIV, COLL MED, DEMENTIA RES SERV, DEPT NEUROL & NEUROSCI, WHITE PLAINS, NY 10605 USA
关键词
vascular smooth muscle cells; intracellular calcium concentration; hypoxic pulmonary vasoconstriction;
D O I
10.1152/ajplung.1997.273.3.L603
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
To investigate mechanisms of inhibition of hypoxic pulmonary vasoconstriction (HPV), we studied pulmonary artery smooth muscle cell (PASMC) responses to hypoxia, utilizing diphenyleneiodonium (DPI), which blocks HPV. We measured cell contraction in primary cultures of rat PASMC grown on collagen gels and cytosolic free Ca2+ concentration ([Ca2+](i)) in PASMC grown on glass. DPI (5 and 20 mu M) caused contraction of PASMC and increased [Ca2+](i). Omission of extracellular Ca2+ diminished the DPI-induced PASMC contraction and greatly reduced the increase in [Ca2+](i). DPI substantially inhibited KCl-induced PASMC contraction (1 mu M DPI) and the increase in [Ca2+](i) (5 mu M DPI). Severe hypoxia contracted PASMC and quadrupled [Ca2+](i). DPI, 1 mu M, substantially inhibited hypoxic contraction, but neither 1 nor 5 mu M DPI diminished the hypoxia-induced increase in [Ca2+](i), which was greatly attenuated by 20 mu M DPI. These data show 1) that DPI increases [Ca2+](i), accounting for DPI-induced PASMC contraction and 2) that 1 and 5 mu M DPI inhibit the hypoxia-induced contraction but not the hypoxia-induced increase in [Ca2+](i), suggesting that DPI inhibits hypoxic PASMC contraction downstream of the Ca2+ signal by desensitizing the contractile apparatus and indicating a potential control point for modulation of HPV.
引用
收藏
页码:L603 / L611
页数:9
相关论文
共 34 条
[1]   INDICATIONS TO AN NADPH OXIDASE AS A POSSIBLE PO2 SENSOR IN THE RAT CAROTID-BODY [J].
ACKER, H ;
DUFAU, E ;
HUBER, J ;
SYLVESTER, D .
FEBS LETTERS, 1989, 256 (1-2) :75-78
[2]   Halothane reduces myofilament Ca2+ sensitivity during muscarinic receptor stimulation of airway smooth muscle [J].
Akao, M ;
Hirasaki, A ;
Jones, KA ;
Wong, GY ;
Bremerich, DH ;
Warner, DO .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1996, 271 (05) :L719-L725
[3]   GLUCOSE-INDUCED ALTERATIONS OF CYTOSOLIC-FREE CALCIUM IN CULTURED RAT TAIL ARTERY VASCULAR SMOOTH-MUSCLE CELLS [J].
BARBAGALLO, M ;
SHAN, J ;
PANG, PKT ;
RESNICK, LM .
JOURNAL OF CLINICAL INVESTIGATION, 1995, 95 (02) :763-767
[4]   CYTOSOLIC FREE CALCIUM AND GENE-EXPRESSION DURING CHEMICAL HYPOXIA [J].
CARROLL, JM ;
TORALBARZA, L ;
GIBSON, G .
JOURNAL OF NEUROCHEMISTRY, 1992, 59 (05) :1836-1843
[5]   MITOSIS OF CONTRACTILE SMOOTH-MUSCLE CELLS IN TISSUE-CULTURE [J].
CHAMLEY, JH ;
CAMPBELL, GR .
EXPERIMENTAL CELL RESEARCH, 1974, 84 (1-2) :105-110
[6]   Gap junctions in vascular tissues - Evaluating the role of intercellular communication in the modulation of vasomotor tone [J].
Christ, GJ ;
Spray, DC ;
ElSabban, M ;
Moore, LK ;
Brink, PR .
CIRCULATION RESEARCH, 1996, 79 (04) :631-646
[7]  
CORNFIELD DN, 1993, AM J PHYSIOL, V265, pL53
[8]   INVOLVEMENT OF AN NAD(P)H OXIDASE AS A PO2 SENSOR PROTEIN IN THE RAT CAROTID-BODY [J].
CROSS, AR ;
HENDERSON, L ;
JONES, OTG ;
DELPIANO, MA ;
HENTSCHEL, J ;
ACKER, H .
BIOCHEMICAL JOURNAL, 1990, 272 (03) :743-747
[9]  
FRIED E, 1994, AM REV RESPIR DIS, V149, pA21
[10]   ANGIOTENSIN-II STIMULATES NADH AND NADPH OXIDASE ACTIVITY IN CULTURED VASCULAR SMOOTH-MUSCLE CELLS [J].
GRIENDLING, KK ;
MINIERI, CA ;
OLLERENSHAW, JD ;
ALEXANDER, RW .
CIRCULATION RESEARCH, 1994, 74 (06) :1141-1148