Creation of superior carboxyfluorescein dyes by blocking donor-excited photoinduced electron transfer

被引：57

作者：

Mineno, Tomoko

Ueno, Tasuku

Urano, Yasuteru

Kojima, Hirotatsu

Nagano, Tetsuo

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

[2] JST Agcy, CREST, Kawagoe, Saitama 3320012, Japan

[3] JST Agcy, PRESTO, Kawagoe, Saitama 3320012, Japan

来源：

ORGANIC LETTERS | 2006年 / 8卷 / 26期

关键词：

D O I：

10.1021/ol0623926

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Carboxyfluoresceins are widely utilized as fluorescence labeling reagents, but we recently found that their emission intensity is markedly decreased after esterification. On the basis of our hypothesis that the fluorescence decrease is due to a donor- excited photoinduced electron transfer (d- PeT) process, we have developed novel carboxyfluorescein derivatives in which the d- PeT process is hampered, and the emission intensity is not decreased upon esterification. These novel dye derivatives display high quantum yields and are expected to be useful as labeling agents.

引用

页码：5963 / 5966

页数：4

共 24 条

[1] COVALENTLY BOUND PH-INDICATOR DYES AT SELECTED EXTRACELLULAR OR CYTOPLASMIC SITES IN BACTERIORHODOPSIN .2. ROTATIONAL ORIENTATION OF HELIX-D AND HELIX-E AND KINETIC CORRELATION BETWEEN M-FORMATION AND PROTON RELEASE IN BACTERIORHODOPSIN MICELLES [J].