Characterisation of an exopolysaccharide preventing phage adsorption in Lactococcus lactis subsp cremoris MA39

被引:0
|
作者
Akçelik, M [1 ]
Sanlibaba, P [1 ]
机构
[1] Ankara Univ, Fac Agr, Dept Food Engn, TR-06110 Ankara, Turkey
来源
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES | 2002年 / 26卷 / 05期
关键词
exopolycaccharide; phage resistance; Lactococcus lactis subsp cremoris;
D O I
暂无
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
Lactococcus lactis subsp. cremoris strain MA39 produces an extracellular polysaccharide containing rhamnose, glucose and galactose. A 16.5 kb plasmid encoding exopolysaccharide production in MA39 was determined by plasmid curing experiments. Phage adsorption assays showed that four different lactococcal phages were adsorbed to MA39-40; only the 16.5 kb plasmid cured an exopolysaccharide non-producing mutant of strain MA39, with high efficiency (93.2-98.5%) while adsorption of these phages was completely inhibited in the wild type strain MA39. These results suggest that exopolysaccharide prevents phage adsorption by masking phage receptor sites.
引用
收藏
页码:1151 / 1156
页数:6
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