Verification of antibody labelling efficiency as an important step in ELISA/QLISA development

被引：10

作者：

Cadkova, Michaela ^{[1
]}

Dvorakova, Veronika ^{[1
]}

Metelka, Radovan ^{[2
]}

Bilkova, Zuzana ^{[1
]}

Korecka, Lucie ^{[1
]}

机构：

[1] Univ Pardubice, Fac Chem Technol, Dept Biol & Biochem Sci, Pardubice, Czech Republic

[2] Univ Pardubice, Fac Chem Technol, Dept Analyt Chem, Pardubice, Czech Republic

来源：

MONATSHEFTE FUR CHEMIE | 2016年 / 147卷 / 01期

关键词：

Biosensors; Immunoassays; Proteins; Alkaline phosphatase; Quantum dots; IMMUNOSENSOR; IMMUNOASSAY; PHOSPHATE;

D O I：

10.1007/s00706-015-1598-1

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A simple and effective method for validation of antibody labelling procedure as well as for optimal conjugate dilution assessment based on electrochemical detection is presented. Enzyme alkaline phosphatase and core-shell CdSe/ZnS quantum dots are used as sensitive tag exploitable as detection tool of antibody-antigen complex in magnetic microbead-based immunoassays. Square-wave voltammetry for detection of the electroactive product that is induced by conversion of appropriate substrate by alkaline phosphatase and square-wave anodic-stripping voltammetry for signal of Cd in case of quantum dots, respectively, was elected. Anti-Apo E IgG antibodies were selected as model molecules for method optimizations. [GRAPHICS] .

引用

页码：69 / 73

页数：5

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