Laminarin-mediated targeting to Dectin-1 enhances antigen-specific immune responses

被引：41

作者：

Xie, Jianhui ^{[1
]}

Guo, Liang ^{[1
]}

Ruan, Yuanyuan ^{[1
]}

Zhu, Haiyan ^{[1
]}

Wang, Lan ^{[1
]}

Zhou, Lei ^{[1
]}

Yun, Xiaojing ^{[1
]}

Gu, Jianxin ^{[1
]}

机构：

[1] Fudan Univ, Shanghai Med Coll, Ctr Gene Res, Shanghai 200032, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 01期

关键词：

Antigen delivery; APCs; Dectin-1; Laminarin; OVA; T-CELL RESPONSES; BETA-GLUCAN RECOGNITION; HUMAN DENDRITIC CELLS; RECEPTOR; INDUCTION; MACROPHAGES; DEC-205; LEADS;

D O I：

10.1016/j.bbrc.2009.11.173

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has immense potential for immunotherapy and vaccination to target antigens to antigen-presenting cells (APCs). Here we described a method for delivering whole protein antigens to APCs via carbohydrate-mediated targeting of Dectin-1, which is a C-type lectin and mainly expresses on subpopulations of dendritic cells and macrophages. Laminarin, which is a beta-1-3 glucan and a typical ligand for Dectin-1, was chemically Coupled to ovalbumin (OVA). Compared to OVA alone, the conjugate was effectively recognized and ingested by CHO cells stably expressing Dectin-1 and bound to bone marrow dendritic cells (BMDCs) via Dectin-1. Laminarin modification led to significant enhancement of OVA-specific CD4(+) T-cell response. Moreover, when used to Immunize mice, the conjugate enhanced the primary IgG antibody response to OVA. Taken together, Our data Suggest that APCs targeting based on glucan-Dectin-1 interaction is a promising approach to improve vaccines. (C) 2009 Elsevier Inc All rights reserved.

引用

页码：958 / 962

页数：5

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