Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases

被引：71
作者：
Ruiz-Moyano, S. [1 ,2 ]
Martin, A. [1 ,2 ]
Villalobos, M. C. [1 ,2 ]
Calle, A. [1 ]
Serradilla, M. J. [3 ]
Cordoba, M. G. [1 ,2 ]
Hernandez, A. [1 ,2 ]
机构：
[1] Univ Extremadura, Escuela Ingn Agr, Nutr & Bromatol, Ave Adolfo Suarez S-N, Badajoz 06007, Spain
[2] Inst Univ Recursos Agr INURA, Ave Invest S-N,Campus Univ, Badajoz 06006, Spain
[3] Ctr Invest Cient & Tecnol Extremadura CICYTEX, Area Vegetales, A5 Km 372, Guadajira 06187, Spain
来源：
FOOD MICROBIOLOGY | 2016年 / 57卷
关键词：
Antagonistic yeast; Hanseniaspora opuntiae; Metschnikowia pulcherrima; Postharvest disease; Botrytis cinerea; Cladosporium cladosporioides; Monilia laxa; Penicillium expansum; BIOLOGICAL-CONTROL; ANTAGONISTIC YEASTS; METSCHNIKOWIA-PULCHERRIMA; PSEUDOMONAS-SYRINGAE; BOTRYTIS-CINEREA; SWEET CHERRIES; PATHOGENS; EFFICACY; VEGETABLES; STRAINS;
D O I：
10.1016/j.fm.2016.01.003
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Fresh fruit is highly perishable during postharvest life, mainly due to fungal growth. Thus, fungal control is an important goal for the fruit industry. In this work, a selection of antagonistic yeasts isolated from fig and breba crops were screened in vitro. The isolated yeasts were challenged with three moulds isolated from decayed figs and breba crops, identified as Penicillium expansum M639 and Cladosporium cladosporioides M310 and M624, and pathogenic moulds Botrytis cinerea CECT20518 and Monilia laxa CA1 from culture collections. Two yeast isolates, Hanseniaspora opuntiae L479 and Metschnikowia pulcherrima L672, were selected for their ability to inhibit the growth of aforementioned moulds. These yeasts reduced the radial growth of moulds on PDA by between 45.23% and 66.09%. Antagonistic activity was associated with the interaction of live yeast cells with moulds. M. pulcherrima L672 apparently parasitised C. cladosporioides isolates. In addition, challenges were assayed using wounded apples and nectarines, with significant reductions in percent infection and lesion size for all moulds tested. To our knowledge, this is the first report identifying H. opuntiae as an antagonist against different pathogenic moulds. (C) 2016 Elsevier Ltd. All rights reserved.
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页码：45 / 53
页数：9
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