Aircraft multi-parameters identification is the one of main approaches for simulating and validating aircraft system in aircraft design, it is very important to accurately reflect performance of flight control system with multiplex system and complexity of aircraft. In order to reveal the authenticity of aircraft, the recursive Least Square Algorithm(RLSA) is presented, therefore, the multi-parameter is recognized in real time and on-line. RLSA is based on recursive process to continually update information, the estimate precision of parameters are improved, especially in adaptive control system of many parameters. The transfer function coefficients of aircraft are identified by using RLSA., it is illustrated that rapid recognition velocity, high identification precision and excessive recognition parameters are possessed. In engineer, it has certain reference and applied values.