Potential False-Positive and False-Negative Results for COVID-19 IgG/IgM Antibody Testing After Heat-Inactivation

被引：5

作者：

Lin, Jie ^{[1
,2
]}

Dai, Wei ^{[1
]}

Li, Weiwei ^{[1
]}

Xiao, Li ^{[1
,3
]}

Luo, Tao ^{[1
]}

Guo, Yanju ^{[1
]}

Yang, Yang ^{[1
]}

Han, Ying ^{[1
]}

Zhu, Peiran ^{[1
]}

Wu, Qiuyue ^{[1
]}

He, Bangshun ^{[1
,4
]}

Wu, Jian ^{[1
]}

Xia, Xinyi ^{[1
,3
]}

机构：

[1] Nanjing Univ, Southern Med Univ, COVID Res Ctr 19, Inst Lab Med,Jinling Hosp,Sch Med,Nanjing Clin Co, Nanjing, Peoples R China

[2] 904 Hosp, Wuxi, Jiangsu, Peoples R China

[3] Wuhan Huoshenshan Hosp, Dept Lab Med & Blood Transfus, Joint Expert Grp COVID 19, Wuhan, Peoples R China

[4] Nanjing Med Univ, Nanjing Hosp 1, Gen Clin Res Ctr, Nanjing, Peoples R China

来源：

FRONTIERS IN MEDICINE | 2021年 / 7卷

关键词：

COVID-19; SARS-CoV-2; IgG and IgM antibody; heat-inactivation; indirect immunity method; CORONAVIRUS; IGM; DIAGNOSIS;

D O I：

10.3389/fmed.2020.589080

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objectives: With the worldwide spread of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), various antibody detection kits have been developed to test for SARS-CoV-2- specific IgG, IgM, and total antibody. However, the use of different testing methods under various heat-inactivation conditions might affect the COVID-19 detection results. Methods: Seven different antibody detection kits produced by four manufacturers for detection of SARS-CoV-2 IgG, IgM, and total antibody were tested at Wuhan Huoshenshan Hospital, China. Most of the kits used the indirect immunity, capture, and double-antigen sandwich methods. The effects of various heat-inactivation conditions on SARS-CoV-2-specific IgG, IgM, and total antibody detection were analyzed for the different test methods. Results: Using the indirect immunity method, values for SARS-CoV-2 IgG antibody significantly increased and those for IgM antibody decreased with increasing temperature of heat-inactivation using indirect immunity method. However, values for SARS-CoV-2 IgM and total antibody showed no change when the capture and double-antigen sandwich methods were used. The changes in IgG and IgM antibody values with the indirect immunity method indicated that heat-inactivation could affect COVID-19 detection results obtained using this method. In particular, 18 (22.2%) SARS-CoV-2 IgM positive samples were detected as negative with heat-inactivation at 65 degrees C for 30 min, and one (25%) IgG negative sample was detected as positive after heat-inactivation at 56 degrees C for 60 min and 60 degrees C for 30 min. Conclusions: Heat-inactivation could increase SARS-CoV-2 IgG antibody values, and decrease IgM antibody values, causing potential false-positive or false-negative results for COVID-19 antibody detection using the indirect immunity method. Thus, before conducting antibody testing, the testing platforms should be evaluated in accordance with the relevant requirements to ensure accurate COVID-19 detection results.

引用

页数：9

共 23 条

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