Early Assessment of Colorectal Cancer by Quantifying Circulating Tumor Cells in Peripheral Blood: ECT2 in Diagnosis of Colorectal Cancer

被引:19
作者
Chen, Chih-Jung [1 ,2 ,3 ]
Sung, Wen-Wei [2 ,3 ,4 ,5 ,6 ]
Chen, Hung-Chang [7 ]
Chern, Yi-Jye [8 ]
Hsu, Hui-Ting [1 ]
Lin, Yueh-Min [1 ,2 ]
Lin, Shu-Hui [1 ,2 ,9 ]
Peck, Konan [8 ]
Yeh, Kun-Tu [1 ,3 ]
机构
[1] Changhua Christian Hosp, Dept Surg Pathol, Changhua 8864, Taiwan
[2] Jen Teh Jr Coll Med Nursing & Management, Dept Med Technol, Miaoli 88637, Taiwan
[3] Chung Shan Med Univ, Sch Med, Taichung 8864, Taiwan
[4] Chung Shan Med Univ Hosp, Dept Urol, Taichung 8864, Taiwan
[5] Chung Shan Med Univ, Inst Med, Taichung 8864, Taiwan
[6] Chung Shan Med Univ Hosp, Dept Med Educ, Taichung 8864, Taiwan
[7] Changhua Christian Hosp, Dept Colon & Rectal Surg, Changhua 8864, Taiwan
[8] Acad Sinica, Inst Biomed Sci, Taipei 8862, Taiwan
[9] Chung Shan Med Univ, Inst Med, Taichuang 8864, Taiwan
来源
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2017年 / 18卷 / 04期
关键词
circulating tumor cell; ECT2; CEA; colorectal cancer; biomarker; RESISTANT PROSTATE-CANCER; COLON-CANCER; PROGRESSION; SURVIVAL; DISSEMINATION; CYTOKINESIS; MARKER; LUNG; EMT; CHEMOTHERAPY;
D O I
10.3390/ijms18040743
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Circulating tumor cells (CTCs) in peripheral blood is an indication of poor prognosis for patients with different cancer types. However, most of the available technologies for detecting CTCs show low sensitivity and specificity. Therefore, we attempted to find an alternative marker for CTCs of colorectal cancer. We have directly extracted RNA from CTCs contained in 1.5 mL peripheral blood from 90 colorectal cancer patients and 151 healthy donors, and screened these samples for candidate marker genes by nested real-time quantitative polymerase chain reaction (PCR). From genes selected from a public database of microarray analyses, we successfully identified epithelial cell transforming sequence 2 oncogene (ECT2) as a gene that exhibits high differential expression ratios (p < 0.01). ECT2 displays good sensitivity and specificity, with an area under the curve (AUC) value of 0.821. This marker gene also has a high detection rate in patients with serum carcinoembryonic antigen (CEA) concentrations below the diagnostic threshold of 5 ng/mL. The expression of ECT2 can therefore serve as an alternative measurement that can compensate for the inadequacy of the current CEA test in the diagnosis and monitoring of colorectal cancer patients.
引用
收藏
页数:11
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